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Effect of the size of evacuated blood collection tubes on total carbon dioxide concentration in equine plasma.
MedLine Citation:
PMID:  23013506     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Objective-To determine whether plasma total CO(2) concentrations would vary with the size of the evacuated tube used to collect blood samples. Design-Randomized crossover study. Animals-Convenience sample of 20 healthy adult horses. Procedures-Jugular venous blood was collected from horses in random order into 8 types of evacuated tubes: 2-mL glass, 2- or 3-mL plastic or plastic plasma separator, 4- or 6-mL plastic, and 10-mL glass or plastic. Total CO(2) concentrations in plasma were measured with a biochemistry analyzer. Data were analyzed via repeated-measures ANOVA and multivariate regression. Results-The air volume-to-blood volume ratio was significantly higher and consequently, plasma total CO(2) concentration was significantly lower when blood was collected into 2-mL glass tubes and 2- or 3-mL plastic tubes than when the other 5 types of evacuated tubes were used. Concentrations in the other tube types were statistically equivalent. A linear relationship was detected between total CO(2) concentration and air volume-to-blood volume ratio. Conclusions and Clinical Relevance-Blood samples should be collected into evacuated tubes with a small air volume-to-blood volume ratio whenever an accurate estimate of plasma total CO(2) concentration is required.
Authors:
Stacy H Tinkler; Laurent L Couëtil; Sarah A Kennedy; Peter D Constable
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of the American Veterinary Medical Association     Volume:  241     ISSN:  1943-569X     ISO Abbreviation:  J. Am. Vet. Med. Assoc.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7503067     Medline TA:  J Am Vet Med Assoc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  922-6     Citation Subset:  IM    
Affiliation:
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN 47907.
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