| Effect of simethicone on the accuracy of the rapid urease test. | |
| | |
MedLine Citation:
|
PMID: 9831407 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: Simethicone, which is a principal ingredient in the defoaming agent used during gastroscopy, can inhibit the growth of Helicobacter pylori (MIC, 64-128 mg/l). This prospective study was designed to evaluate the in vivo and in vitro effects of simethicone on the accuracy of the rapid urease test (RUT). METHODS: In the in vivo study, three sets of gastric biopsies (two from the antrum, and one from the corpus) were taken from 75 patients. The first set was examined histologically, and the second set was used for the RUT (pre-simethicone RUT). Then, 25 ml simethicone (1200 mg/l) was introduced into the stomach for a contact time of 1 min. A third set of gastric biopsies was taken for the RUT (post-simethicone RUT). In the in vitro clinical study, 41 patients were recruited. The first set of gastric biopsies was used for the RUT (pre-incubation RUT). The second set was incubated in 1 ml of simethicone for 5 min before being used for the RUT (post-incubation RUT). In the spectrophotometric study, urease activity before and after incubation in simethicone for 5 min was quantified in 12 patients by measuring the absorbance at 560 nm. RESULTS: Reading at 15 min, the concordance rate between the pre-simethicone and post-simethicone RUT was 98%. In the in vitro clinical study, the concordance rate between the pre-incubation and post-incubation RUT was 97%. The spectophotometric study showed a significant reduction of 43% in urease activity after incubation in simethicone. CONCLUSION: The application of a defoaming agent containing simethicone does not affect the accuracy of the RUT. However, simethicone modestly suppresses urea hydrolysis. |
| | |
Authors:
|
F H Ng; S Y Wong; C Kng; S L Chow; K C Lai; W F Ng |
Related Documents
:
|
19046017 - Effects of intravenous administration of caffeine on physiologic variables in exercisin... 6869347 - Effects of a meal on the motility of the sigmoid colon and rectum in healthy adults. 8536157 - Sham feeding disrupts phase iii of the duodenal migrating motor complex in humans. 15253087 - Radiographic evaluation of sclerosis of the third carpal bone associated with exercise ... 21393257 - Fetal wellbeing may be compromised during strenuous exercise among pregnant elite athle... 18417547 - Exercise-induced protection against myocardial apoptosis and necrosis: mnsod, calcium-h... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: European journal of gastroenterology & hepatology Volume: 10 ISSN: 0954-691X ISO Abbreviation: Eur J Gastroenterol Hepatol Publication Date: 1998 Oct |
Date Detail:
|
Created Date: 1999-01-27 Completed Date: 1999-01-27 Revised Date: 2009-10-16 |
Medline Journal Info:
|
Nlm Unique ID: 9000874 Medline TA: Eur J Gastroenterol Hepatol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 851-4 Citation Subset: IM |
Affiliation:
|
Department of Medicine, Ruttonjee Hospital, Wan Chai, Hong Kong, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Diagnostic Techniques, Digestive System Helicobacter Infections / diagnosis Helicobacter pylori / drug effects, enzymology, isolation & purification Humans Prospective Studies Sensitivity and Specificity Simethicone / pharmacology* Stomach / drug effects*, enzymology*, microbiology Urease / metabolism* |
| Chemical | |
Reg. No./Substance:
|
8050-81-5/Simethicone; EC 3.5.1.5/Urease |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Continuous and more effective duodenal ulcer healing under therapy with bismuth and two antibiotics ...
Next Document: Variable distribution of Cryptosporidium parvum in the intestine of AIDS patients revealed by polyme...