| Effect of short-term morphine exposure on P-glycoprotein expression and activity in cancer cell lines. | |
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MedLine Citation:
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PMID: 15069552 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Multidrug resistance (MDR) is a common problem in various types of cancer. One important factor in the development of MDR is overexpression of P-glycoprotein, encoded by the MDR1 gene. Morphine is the opioid of choice for moderate to severe cancer pain, and is a substrate of P-glycoprotein. Recently, morphine has been shown to induce P-glycoprotein expression in the rat brain. Using Western blot analysis and cytotoxicity assays respectively, we have investigated the effects of short-term (72 h) morphine treatment on P-glycoprotein expression in a panel of human cancer cell lines, and its effects on cellular resistance to the known P-glycoprotein substrates, vinblastine and colchicine. The effect of morphine on P-glycoprotein expression and activity in the mouse fibroblast NIH-3T3 cell was assessed to establish whether morphine effects are species specific. Short-term exposure to morphine did not result in any significant differences in P-glycoprotein expression or activity in any cancer cell lines. Morphine pre-treatment resulted in a moderate but significant increase in sensitivity of NIH-3T3 cells to vinblastine, but not colchicine. This study suggests that morphine effects may be cell-type specific. Importantly, however, it appears that short-term morphine treatment does not affect the MDR phenotype of tumour cells. |
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Authors:
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Marina Pajic; Mary Bebawy; Janelle M Hoskins; Basil D Roufogalis; Laurent P Rivory |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Oncology reports Volume: 11 ISSN: 1021-335X ISO Abbreviation: Oncol. Rep. Publication Date: 2004 May |
Date Detail:
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Created Date: 2004-04-07 Completed Date: 2004-11-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9422756 Medline TA: Oncol Rep Country: Greece |
Other Details:
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Languages: eng Pagination: 1091-5 Citation Subset: IM |
Affiliation:
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Children's Cancer Institute of Australia for Medical Research, Randwick, Sydney, NSW 2031, Australia. mpajic@ccia.org.au |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line, Tumor Cell Survival / drug effects Colchicine / toxicity Dose-Response Relationship, Drug Gene Expression Regulation, Neoplastic / drug effects* Humans Mice Morphine / pharmacology* P-Glycoprotein / metabolism* Vinblastine / toxicity |
| Chemical | |
Reg. No./Substance:
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0/P-Glycoprotein; 57-27-2/Morphine; 64-86-8/Colchicine; 865-21-4/Vinblastine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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