Document Detail

Effect of shear stress on the expression of coagulation and fibrinolytic factors in both smooth muscle and endothelial cells in a co-culture model.
MedLine Citation:
PMID:  18303268     Owner:  NLM     Status:  MEDLINE    
METHODS: A co-culture of human saphenous vein EC (HSVEC) and human saphenous vein SMC (HSVSMC) was exposed to shear, following which the cells were separated. Gene expression of tissue factor, thrombomodulin (TM), plasminogen activator inhibitor-1 (PAI-1), tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA) were analyzed with real-time RT-PCR. Protein expression was studied with ELISA. In HSVEC, the expression of PAI-1 (x2.1), tPA (x1.8), uPA (x1.6), tissue factor (x2.5) and TM (x1.9) was upregulated after 4 h of shear compared to controls. After 24 h of shear, expression was still upregulated in tPA (x2.3) and TM (x1.6). In HSVSMC, change in expression of PAI-1 (x2.1) was present after 4 h and in uPA (x2.1), and TM (x0.4) after 24 h. Both HSVEC and HSVSMC responded to shear, which led to altered expression of coagulation and fibrinolytic factors. This indicates that SMC, and interactions between EC and SMC, are more important in the regulation of vascular wall hemostasis than earlier studies have reported.
G Helenius; S H Hagvall; M Esguerra; H Fink; R Soderberg; B Risberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-26
Journal Detail:
Title:  European surgical research. Europäische chirurgische Forschung. Recherches chirurgicales européennes     Volume:  40     ISSN:  1421-9921     ISO Abbreviation:  Eur Surg Res     Publication Date:  2008  
Date Detail:
Created Date:  2008-05-19     Completed Date:  2008-07-17     Revised Date:  2013-05-20    
Medline Journal Info:
Nlm Unique ID:  0174752     Medline TA:  Eur Surg Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  325-32     Citation Subset:  IM    
Copyright Information:
2008 S. Karger AG, Basel.
Vascular Engineering Centre, Department of Surgery, Sahlgrenska University Hospital, Goteborg, Sweden.
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MeSH Terms
Blood Coagulation / physiology*
Cells, Cultured
Coculture Techniques
Endothelial Cells / metabolism*
Myocytes, Smooth Muscle / metabolism*
Plasminogen Activator Inhibitor 1 / metabolism
Stress, Mechanical
Thrombomodulin / metabolism
Thromboplastin / metabolism
Tissue Plasminogen Activator / metabolism
Urokinase-Type Plasminogen Activator / metabolism
Reg. No./Substance:
0/Plasminogen Activator Inhibitor 1; 0/Thrombomodulin; 9035-58-9/Thromboplastin; EC Plasminogen Activator; EC Plasminogen Activator

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