| Effect of serum amyloid A1 treatment on global gene expression in THP-1-derived macrophages. | |
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MedLine Citation:
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PMID: 22228103 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the effect of serum amyloid A1 (SAA1) on global gene expression in macrophages derived from THP-1 monocytes. MATERIALS AND METHODS: Global genetic expression in THP-1-derived macrophages was determined using Illumina HT-12 microarray chips and the results were validated by real-time PCR. Cytokine levels in cellular supernatant were quantified by ELISA. RESULTS: In total, 55 genes were upregulated with fold difference greater than two when THP-1-derived macrophages were incubated with SAA1 for 8 h. SAA1 is a strong cytokine inducer with significant upregulation of chemokines CCL1, CCL3, and CCL4 and this was confirmed by both real-time PCR and ELISA quantification. SAA1 also promotes the upregulation of genes involved in phagocytosis, anti-apoptosis, and tissue remodeling. CONCLUSIONS: SAA1 appears to play an important role during the immune response and in chronic inflammatory diseases through the stimulation of genes involved in cytokine production, phagocytosis, and anti-apoptosis. |
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Authors:
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Koon-Yeow Leow; Wilson Wen Bin Goh; Chew-Kiat Heng |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-8 |
Journal Detail:
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Title: Inflammation research : official journal of the European Histamine Research Society ... [et al.] Volume: - ISSN: 1420-908X ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9508160 Medline TA: Inflamm Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, 1E, Kent Ridge Road, Singapore, 119228, Singapore. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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