Document Detail


Effect of sepsis and 3,5,3'-triiodothyronine replacement on myocardial integrity during oxidant challenge.
MedLine Citation:
PMID:  8706464     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To determine whether sepsis, with or without thyroid hormonal augmentation, induces myocardial tolerance to an oxidant challenge. DESIGN: A prospective, randomized, controlled animal trial. SETTING: University research laboratory. SUBJECTS: Twenty male Sprague-Dawley rats. INTERVENTIONS: After anesthesia, animals underwent cecal ligation and puncture, with or without 3,5,3'-triiodothyronine replacement (3 ng/hr), or sham surgery. Twenty-four hours later, the heart was rapidly excised for retrograde Langendorff perfusion. Oxyradical challenge consisted of the addition of 200 microM of hydrogen peroxide to the perfusate for 60 mins. MEASUREMENTS AND MAIN RESULTS: Myocardial contractility and relaxation were continuously recorded. Perfusate glutathione and lactate dehydrogenase concentrations were determined enzymatically at 30-min intervals for 90 mins. Oxyradical perfusion alone significantly increased glutathione efflux and decreased myocardial contractility when compared with control animals. Prior cecal ligation and puncture decreased oxidant-mediated glutathione efflux and maintained myocardial contractility. 3,5,3'-triiodothyronine supplementation appeared to increase late cardiac contractility and cellular integrity during oxidant challenge. However, this increase was not statistically significant. CONCLUSIONS: Antecedent septic challenge appears to induce tolerance to further myocardial oxyradical exposure and improves myocardial functional and biochemical integrity. Thyroid hormonal supplementation may provide a modest additional benefit in septic animals.
Authors:
S B Davidson; S A Dulchavsky; L N Diebel; S Dutta; W J Cullen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Critical care medicine     Volume:  24     ISSN:  0090-3493     ISO Abbreviation:  Crit. Care Med.     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-09-10     Completed Date:  1996-09-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0355501     Medline TA:  Crit Care Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  850-4     Citation Subset:  AIM; IM    
Affiliation:
Department of Surgery, Wayne State University School of Medicine, Detroit, MI, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Disease Models, Animal
Drug Evaluation, Preclinical
Glutathione / metabolism
L-Lactate Dehydrogenase / metabolism
Lipid Peroxidation
Male
Myocardial Contraction / drug effects*
Myocardium / metabolism*
Oxidants / adverse effects*
Prospective Studies
Random Allocation
Rats
Rats, Sprague-Dawley
Sepsis / complications*,  drug therapy*,  metabolism
Triiodothyronine / analogs & derivatives*,  therapeutic use
Chemical
Reg. No./Substance:
0/Oxidants; 51-24-1/3,3',5-triiodothyroacetic acid; 6893-02-3/Triiodothyronine; 70-18-8/Glutathione; EC 1.1.1.27/L-Lactate Dehydrogenase

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