| Effect of selenium intake and fetal age on mRNA levels of two selenoproteins in porcine fetal and maternal liver. | |
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MedLine Citation:
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PMID: 16908641 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this study was to determine if levels of mRNA encoding cytosolic glutathione peroxidase (cGPx) and thioredoxin reductase (TrxR-1) change during fetal development, and if maternal Se intake during gestation affects the mRNA levels of these proteins. Prepubertal gilts (n = 24) were randomly assigned to either Se-adequate (0.39 ppm of Se; n = 12) or Se-deficient (0.05 ppm of Se; n = 12) diets, 6 wk before breeding. Maternal liver was collected at d 10, 45, 70, and 114 of pregnancy, and fetal liver samples were collected at the same times except d 10. Complementary DNA sequences encoding cGPx and TrxR-1 were cloned and sequenced. Quantitative real-time PCR analysis indicated that levels of mRNA for cGPx in fetal liver decreased more than 3-fold between d 45 and 114 of gestation. Although the gilts were only marginally deficient in Se, and maternal Se intake did not affect cGPx mRNA levels in fetal liver, the low-Se diet tended (P = 0.1) to reduce fetal TrxR-1 mRNA levels. In the liver of the dams, the low Se intake did not affect mRNA levels for either cGPx or TrxR-1. Compared with the liver of the dams, mRNA levels for cGPx were about 3.5 times lower in fetal liver. Results of this study support the hypothesis that neonatal pigs are born with reduced cGPx corresponding to reduced cGPx mRNA levels during late gestation. |
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Authors:
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C E Hostetler; J Michal; M Robison; T L Ott; R L Kincaid |
Publication Detail:
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Type: Journal Article; Randomized Controlled Trial |
Journal Detail:
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Title: Journal of animal science Volume: 84 ISSN: 1525-3163 ISO Abbreviation: J. Anim. Sci. Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-08-15 Completed Date: 2006-10-25 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8003002 Medline TA: J Anim Sci Country: United States |
Other Details:
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Languages: eng Pagination: 2382-90 Citation Subset: IM |
Affiliation:
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School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4660, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed Animal Nutritional Physiological Phenomena Animals Base Sequence Diet / veterinary Female Fetus / drug effects, metabolism* Gene Expression Regulation, Developmental / drug effects, genetics* Gestational Age Glutathione Peroxidase / genetics* Liver / drug effects, metabolism* Pregnancy RNA, Messenger / chemistry, metabolism Selenium / administration & dosage, pharmacology* Swine / metabolism* Thioredoxin-Disulfide Reductase / genetics* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 7782-49-2/Selenium; EC 1.11.1.9/Glutathione Peroxidase; EC 1.8.1.9/Thioredoxin-Disulfide Reductase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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