Document Detail

Effect of selenium on expression of selenoproteins in mouse fibrosarcoma cells.
MedLine Citation:
PMID:  15894816     Owner:  NLM     Status:  MEDLINE    
Selenium (Se), an essential trace element, is incorporated into selenoproteins as selenocysteine using insertion machinery, including UGA codon and selenocysteine insertion sequence (SECIS) element in the 3'-untranslated region (3'-UTR) of mRNA. To assess the biological effects of tumor cells exposed to the elevated, but nontoxic Se level on glutathione peroxidase (GPx1 [cellular] and GPx3 [extracellular]), thioredoxin reductase (TrxR), and selenoprotein P (SeP) mRNA expression, we introduced a semiquantitative reverse transcription-polymerase chain reaction technique for each selenoprotein transcript using beta-actin as a reference housekeeping gene in mouse fibroblasts (WEHI 164). Cell lines were cultured with 1.0, 2.5, and 5.0 ng of Se in 1 mL of medium for 3 and 7 d, apart from the control cell line with standard medium. It was found that Se exerts a statistically significant (p<0.05) effect only on GPx3 mRNA, referred to as the optical density (OD) ratio (GPx3/beta-actin). Moreover, the lowest Se level affected GPx3 mRNA expression more strongly than its highest concentrations. In an in vitro model applied in this study, GPx3 gene expression is most specific for Se supplementation.
Edyta Reszka; Jolanta Gromadzinska; Malgorzata Stanczyk; Wojciech Wasowicz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological trace element research     Volume:  104     ISSN:  0163-4984     ISO Abbreviation:  Biol Trace Elem Res     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-16     Completed Date:  2005-08-29     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7911509     Medline TA:  Biol Trace Elem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  165-72     Citation Subset:  IM    
Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Lodz, Poland.
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MeSH Terms
Actins / biosynthesis
Gene Expression / drug effects
Glutathione Peroxidase / biosynthesis*
Proteins / metabolism*
Selenium / pharmacology*
Selenocysteine / metabolism
Selenoprotein P
Thioredoxin Reductase 1
Thioredoxin-Disulfide Reductase / biosynthesis
Tumor Cells, Cultured
Reg. No./Substance:
0/Actins; 0/Proteins; 0/Selenoprotein P; 0/Selenoproteins; 10236-58-5/Selenocysteine; 7782-49-2/Selenium; EC 1.11.1.-/Gpx3 protein, mouse; EC 1.11.1.-/glutathione peroxidase GPX1; EC Peroxidase; EC Reductase 1; EC Reductase; EC protein, mouse

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