Document Detail


Effect of selected insecticides on growth rate and stress protein expression in cultured human A549 and SH-SY5Y cells.
MedLine Citation:
PMID:  16884889     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two organochlorines (dienochlor, endosulfan) and one neonicotinoid (imidacloprid) insecticides were investigated as putative cellular aggressors, both as pure chemicals and as commercial formulations, in order to evaluate the additional toxicity due to additives present in the commercial formulations. Toxicity was evaluated on human cells in vitro, by culturing neuronal SH-SY5Y and pulmonary A549 cell lines for 3 days in the presence of increasing concentrations of the selected pesticides. LOEC (lowest observed effect concentration), IC50 (concentration leading to a 50% decrease of cell growth) and expression changes of molecular chaperones involved in cellular protein quality control were determined. The investigated molecular chaperones were the cytosolic resident heat shock proteins (HSP27, HSP72/73, and HSP90) and the glucose regulated proteins (GRP78, GRP94) located in the endoplasmic reticulum (ER). Organochlorines were found to be the most toxic in both A549 and SH-SY5Y cells, IC50 being respectively 0.95 and 0.36 microM for dienochlor, 34 and 20 microM for endosulfan, 1.8 and 1.5 mM for imidacloprid. This shows that neuronal cells were more sensitive than pulmonary cells. LOEC and IC50 appeared at lower concentrations of active molecule when using the commercial formulations Techn'ufan (endosulfan) and Confidor (imidacloprid), indicating an additional adverse effect of additives. Insecticide concentrations higher than IC50 were found to induce an underexpression of all cytosolic HSPs, probably resulting from a general inhibition of protein synthesis. HSP27 was found to be underexpressed at concentrations of imidacloprid or endosulfan (as Techn'ufan) lower than IC50. This underexpression of the anti-apoptotic HSP27 could contribute to the increase of cell mortality. GRP78 was up-regulated by endosulfan in A549, but not in SH-SY5Y cells, suggesting a damaging effect on proteins specific to pulmonary cells. Conversely, HSP72/73 was found to be down-regulated, resulting probably from the ER unfolded protein response (UPR) as previously reported [Skandrani, D., Gaubin, Y., Vincent, C., Beau, B., Murat, J.C., Soleilhavoup, J.P., Croute, F., 2006. Relationship between toxicity of selected insecticides and expression of stress protein (HSP, GRP) in cultured human cells: effects of commercial formulations versus pure active molecules. Biochim. Biophys. Acta 1760 (1), 95-103].
Authors:
D Skandrani; Y Gaubin; B Beau; J C Murat; C Vincent; F Croute
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-06-29
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  20     ISSN:  0887-2333     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-10-18     Completed Date:  2006-12-26     Revised Date:  2009-04-10    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  1378-86     Citation Subset:  IM    
Affiliation:
Laboratoire de Biologie Cellulaire et Pollution, Faculté de Médecine-Purpan, Université Toulouse-III, 37 allées Jules Guesde, 31073 Toulouse cedex, France.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / pathology
Blotting, Western
Cell Line
Cell Proliferation / drug effects
Endosulfan / toxicity
Glucose / metabolism
Heat-Shock Proteins / biosynthesis*,  metabolism
Humans
Hydrocarbons, Chlorinated / toxicity
Imidazoles / toxicity
Insecticides / toxicity*
Kinetics
Lung Neoplasms / pathology
Molecular Chaperones / metabolism
Nitro Compounds
Protein Folding
Chemical
Reg. No./Substance:
0/Heat-Shock Proteins; 0/Hydrocarbons, Chlorinated; 0/Imidazoles; 0/Insecticides; 0/Molecular Chaperones; 0/Nitro Compounds; 105827-78-9/imidacloprid; 115-29-7/Endosulfan; 2227-17-0/dienochlor; 50-99-7/Glucose

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