Document Detail


Effect of sediment turbidity and color on light output measurement for Microtox Basic Solid-Phase Test.
MedLine Citation:
PMID:  15910896     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this work, sediment samples collected from several Spanish harbours were tested with two toxicity procedures, designed for solid samples: the Microtox Basic Solid-Phase Test (BSPT) and a modified procedure of the previous test protocol (mBSPT). According to the BSPT procedure, after initial light readings, pure bacteria were exposed to sediment suspension dilutions and light production was directly measured on suspended sediments without any further manipulation. As measurements are likely to be affected by sediment turbidity and color, a variation in initial light measurement has been here suggested, in order to consider the sample effect at all time readings during the test. Firstly, when sediment suspensions at different concentrations were added to bacteria suspension, immediately the initial light output drastically decayed by more than 50% in signal difference, resulting in a false inhibition, as effect of sample turbidity/color. This effect was more evident at high EC50 values, when slightly or not toxic samples were assessed. Secondly, the comparison of the EC50 obtained with both procedures, demonstrated that the mBSPT produced higher EC50 values (less toxic) than those obtained with the standard procedure. The mBSPT procedure resulted rapid and effective and it could be applied simultaneously with BSPT, in order to better evaluate the toxicity.
Authors:
T Campisi; F Abbondanzi; C Casado-Martinez; T A DelValls; R Guerra; A Iacondini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemosphere     Volume:  60     ISSN:  0045-6535     ISO Abbreviation:  Chemosphere     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-05-24     Completed Date:  2005-07-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0320657     Medline TA:  Chemosphere     Country:  England    
Other Details:
Languages:  eng     Pagination:  9-15     Citation Subset:  IM    
Affiliation:
Centro Ricerche e Servizi Ambientali Fenice, via C. Menotti, 48 I-48023 Marina di Ravenna (RA), Italy.
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Biological Assay / instrumentation*
Color
Geologic Sediments / analysis*
Light
Linear Models
Nephelometry and Turbidimetry
Seawater / analysis
Software
Spain
Vibrio / drug effects*
Water Microbiology
Water Pollutants, Chemical / analysis,  toxicity*
Chemical
Reg. No./Substance:
0/Water Pollutants, Chemical

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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