Document Detail


Effect of scopoletin on PC3 cell proliferation and apoptosis.
MedLine Citation:
PMID:  11749777     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate the effect of scopoletin on cell proliferation and apoptosis of PC3 cells. METHODS: Cell growth curve, MTT assay, and acid phosphatase activity (ACP) were used to determine cell proliferation. Coomassie brilliant blue assay was used to measure the content of protein in cells. Light microscope, transmission electronmicroscope, and fluorescence microscope were used to observe scopoletin-induced morphological changes. Apoptosis rate and cell cycle distribution were determined by flow cytometry. RESULTS: The IC50 of scopoletin for inhibiting PC3, PAA, and Hela cell proliferation was (157 +/- 25), (154 +/- 51), and (294 +/- 100) mg/L, respectively. Scopoletin induced a marked time- and concentration-dependent inhibition of PC3 cell proliferation. Scopoletin reduced the protein content and decreased the ACP level in PC3 cells in a concentration-dependent manner. Cells treated by scopoletin showed typical morphologic changes of apoptosis by light microscope, fluorescence microscope, and transmission electronmicroscope. Apoptosis rate was 0.3 %, 2.1 %, 9.3 % and 35 % for scopoletin 0, 100, 200, and 400 mg/L, respectively, and cells in G2 phase decreased markedly after being treated with scopoletin. CONCLUSION: Scopoletin inhibited PC3 proliferation by inducing apoptosis of PC3 cells.
Authors:
X L Liu; L Zhang; X L Fu; K Chen; B C Qian
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta pharmacologica Sinica     Volume:  22     ISSN:  1671-4083     ISO Abbreviation:  Acta Pharmacol. Sin.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-12-25     Completed Date:  2003-10-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100956087     Medline TA:  Acta Pharmacol Sin     Country:  China    
Other Details:
Languages:  eng     Pagination:  929-33     Citation Subset:  IM    
Affiliation:
Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China. liuxueli@telekbird.com.cn
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Cell Division / drug effects
Hela Cells / pathology
Humans
Lung Neoplasms / pathology
Male
Prostatic Neoplasms / pathology*
Scopoletin / pharmacology*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
92-61-5/Scopoletin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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