Document Detail


Effect of salt concentration in the recovery medium on heat-injured Streptococcus faecalis.
MedLine Citation:
PMID:  4968964     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Properties relating to the recovery of three heat-injured strains of Streptococcus faecalis were studied. All strains were cultured in all purpose plus Tween broth (APT) at 30 C for 24 hr before being subjected to heat in fresh APT broth. APT recovery medium containing various added amounts of NaCl, KCl, MgCl(2), or KCl and MgCl(2) was used to assess the effect of salts on the recovery of thermally injured S. faecalis. It was evident that, upon exposure to heat, S. faecalis cells became sensitive to increased salt concentrations. Analyses to determine the ribonucleic acid (RNA) content of heated cells showed a reduction of cellular RNA, but the per cent reduction was not directly proportional to the per cent reduction of the viable cells.
Authors:
L R Beuchat; R V Lechowich
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  16     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1968 May 
Date Detail:
Created Date:  1968-08-26     Completed Date:  1968-08-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  772-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Culture Media
Enterococcus faecalis / analysis,  drug effects*
Hot Temperature
Magnesium / pharmacology*
Potassium Chloride / pharmacology*
RNA, Bacterial / analysis
Sodium Chloride / pharmacology*
Chemical
Reg. No./Substance:
0/Culture Media; 0/RNA, Bacterial; 7439-95-4/Magnesium; 7447-40-7/Potassium Chloride; 7647-14-5/Sodium Chloride
Comments/Corrections

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