Document Detail


Effect of salivary proteins on the transport of tannin and quercetin across intestinal epithelial cells in culture.
MedLine Citation:
PMID:  16890919     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polyphenols including tannins and flavonoids are common in plant foods. While tannins may be deleterious to animals, flavonoids can have beneficial effects on the cardio-vascular system. Since salivary proteins can form complexes with polyphenols and thereby interfere with their intestinal absorption, the effect of salivary proteins on transport of tannins and flavonoids across intestinal epithelial cells (Caco-2 cells) was investigated. In presence of the salivary protein Histatin5 (Hst5) the rate of transport (dC/dt) of pentagalloyl glucose (5GG), a tannic acid, across Caco-2 cells in culture was reduced maximally 3.6-fold to 5x10(-7)microM/s in apical to basolateral direction. Replacing the basolateral salt solution with serum caused a 13-fold increase in dC/dt of 5GG in the absence of Hst5, but addition of Hst5 decreased dC/dt l4-fold. Transport of 5GG in basolateral to apical direction was decreased in half in the presence of Hst5. Decreases in dC/dt were closely paralleled by formation of insoluble 5GG-Hst5 complexes. In contrast, Hst5 and 1B4, a salivary proline-rich protein, had little if any effect on the transepithelial transport of the flavonoid quercetin in apical to basolateral as well as basolateral to apical direction. Taken together with previous studies [Cai K, Hagerman AE, Minto RE, Bennick A. Decreased polyphenol transport across cultured intestinal cells by a salivary proline-rich protein. Biochem Pharmacol 2006;71:1570-80] it appears that although Hst5 and 1B4 are synthesized in salivary glands they have an important biological function in the intestines as scavenger molecules preventing uptake of tannin but without notably affecting absorption of flavonoids.
Authors:
Kuihua Cai; Anders Bennick
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-08-07
Journal Detail:
Title:  Biochemical pharmacology     Volume:  72     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-19     Completed Date:  2006-11-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  974-80     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Medical Science Building, University of Toronto, 1 King's College Circle, Toronto M5S 1A8, Canada.
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MeSH Terms
Descriptor/Qualifier:
Biological Transport / drug effects
Caco-2 Cells
Epithelial Cells / drug effects,  metabolism
Histatins
Humans
Hydrolyzable Tannins / metabolism*
Intestinal Absorption / drug effects
Intestines / metabolism*
Quercetin / metabolism*
Salivary Proteins and Peptides / pharmacology*
Chemical
Reg. No./Substance:
0/HTN3 protein, human; 0/Histatins; 0/Hydrolyzable Tannins; 0/Salivary Proteins and Peptides; 0/pentagalloylglucose; 117-39-5/Quercetin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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