Document Detail


Effect of salinity on osmoregulatory patch epithelia in gills of the blue crab Callinectes sapidus.
MedLine Citation:
PMID:  16641518     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In euryhaline crabs, ion-transporting cells are clustered into osmoregulatory patches on the lamellae of the posterior gills. To examine changes in the branchial osmoregulatory patch in the blue crab Callinectes sapidus in response to change in salinity and to correlate these changes with other osmoregulatory responses, crabs were acclimated to a range of salinities between 10 and 35 ppt. When crabs that had been acclimated to 35 ppt were subsequently transferred to 10 ppt, both the size of the osmoregulatory patch on individual gill lamellae and the specific activity of Na+, K+-ATPase in whole-gill homogenates increased only after the first 24 h of exposure to dilute seawater. Enzyme activity and size of patch area increased gradually and reached their maxima (increasing by 200% and 60%, respectively) 6 days following transfer to 10 ppt seawater and then remained at these levels. Patch size at acclimation varied inversely with the salinity for seawater dilutions below 26 ppt (the isosmotic point of the crab), although it did not vary in salinities at or above 26 ppt. Thus, the size of the patch clearly is modulated with acclimation salinity, but it increases only in those salinities in which the crab hyperosmoregulates. An increase in the total RNA/DNA ratio in gill homogenates, the lack of mitotic figures in the lamellae, and the lack of incorporation of bromodeoxyuridine into nuclei of lamellar epithelial cells during acclimation to dilute seawater were interpreted as evidence that no cell proliferation had occurred and that increases in the size of the osmoregulatory patch occurred through differentiation of existing gas exchange cells or of undifferentiated epithelial cells into ion-transporting cells.
Authors:
D L Lovett; T Colella; A C Cannon; D H Lee; A Evangelisto; E M Muller; D W Towle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Biological bulletin     Volume:  210     ISSN:  0006-3185     ISO Abbreviation:  Biol. Bull.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-27     Completed Date:  2007-03-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984727R     Medline TA:  Biol Bull     Country:  United States    
Other Details:
Languages:  eng     Pagination:  132-9     Citation Subset:  IM    
Affiliation:
Department of Biology, The College of New Jersey, Ewing, New Jersey 08628, USA. lovett@tcnj.edu
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological
Animals
Brachyura / anatomy & histology,  metabolism*
Epithelium / anatomy & histology,  drug effects*,  metabolism
Gills / anatomy & histology,  drug effects*,  metabolism*
Male
Seawater
Sodium Chloride / pharmacology*
Time Factors
Water-Electrolyte Balance / physiology*
Chemical
Reg. No./Substance:
7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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