Document Detail


Effect of salicylhydroxamic acid on endosperm strength and embryo growth of Lactuca sativa L. cv Waldmann's Green seeds.
MedLine Citation:
PMID:  11538237     Owner:  NASA     Status:  MEDLINE    
Abstract/OtherAbstract:
Salicylhydroxamic acid (SHAM) stimulated germination of photosensitive lettuce (Lactuca sativa L. cv Waldmann's Green) seeds in darkness. To determine whether SHAM acts on the embryo or the endosperm, we investigated separately effects of SHAM on growth potential of isolated embryos as well as on endosperm strength. Embryo growth potential was quantified by incubating decoated embryos in various concentrations of osmoticum and measuring subsequent radicle elongation. Growth potential of embryos isolated from seeds pretreated with 4 millimolar SHAM was equal to that of untreated controls. Rupture strength of endosperm tissue excised from seeds pretreated with SHAM was 33% less than that of controls in the micropylar region. To determine if the embryo must be in contact with the endosperm of SHAM to weaken the endosperm, some endosperms were incubated with SHAM only after dissection from seeds. Rupture strength of SHAM-treated, isolated endosperms in the micropylar region was 25% less than that of untreated controls. There was no difference in rupture strength in the cotyledonary region of endosperm isolated from seeds treated with SHAM in buffer or buffer alone. SHAM therefore stimulates germination not by enhancing embryo growth potential, but by weakening the micropylar region of the endosperm enclosing the embryo.
Authors:
C A Brooks; C A Mitchell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  86     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1988  
Date Detail:
Created Date:  1996-12-16     Completed Date:  1996-12-16     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  826-9     Citation Subset:  S    
Affiliation:
Department of Horticulture, Purdue University, West Lafayette, Indiana 47907, USA.
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MeSH Terms
Descriptor/Qualifier:
Darkness
Germination / drug effects*,  physiology
Hydrogen-Ion Concentration
Lettuce / drug effects,  embryology*,  growth & development
Salicylamides / pharmacology*
Seeds / drug effects,  growth & development*
Chemical
Reg. No./Substance:
0/Salicylamides; 89-73-6/salicylhydroxamic acid
Investigator
Investigator/Affiliation:
C A Mitchell / Purdue U, West Lafayette, IN
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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