Document Detail


Effect of rotavirus infection on lipid composition and glucose uptake in infant mouse intestine.
MedLine Citation:
PMID:  11206867     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To study the effect of rotavirus infection on lipid composition and glucose uptake in small intestine of infant mice. METHODS: Thirty six 7-day old balb/c mice were given 50 mL (100 ID50) rotavirus suspension orally; 36 control animals received only normal saline. Body weight of animals was recorded. Six animals in each group were sacrificed on days 0, 1, 3, 5, 7 and 10 post-inoculation (pi). Intestines were removed, everted and homogenized in ice-cold saline. Cholesterol and phospholipid content, glucose uptake and alkaline phosphatase in the jejunum and ileum were determined. RESULTS: Rotavirus infection led to a significant decrease in body weight with on days 3 and 5 pi as compared to controls, and to an increase in cholesterol and phospholipid content of the intestine. Uptake of glucose also increased significantly. Alkaline phosphatase activity was significantly reduced on days 3 and 5 pi as compared to controls. CONCLUSION: This study provides characterization of the lipid composition and uptake of glucose in infant mice small intestinal segments during rotavirus infection.
Authors:
R Katyal; S Rana; K Vaiphei; S Ojha; V Singh; K Singh
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology     Volume:  20     ISSN:  0254-8860     ISO Abbreviation:  Indian J Gastroenterol     Publication Date:    2001 Jan-Feb
Date Detail:
Created Date:  2001-02-06     Completed Date:  2001-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8409436     Medline TA:  Indian J Gastroenterol     Country:  India    
Other Details:
Languages:  eng     Pagination:  18-21     Citation Subset:  IM    
Affiliation:
Department of Gastroenterology, Postgraduate Institute of Medical Education and Research, Chandigarh.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Disease Models, Animal
Female
Glucose / metabolism*
Intestinal Absorption / physiology*
Jejunum / metabolism*,  virology
Male
Mice
Mice, Inbred BALB C
Phospholipids / metabolism*
Probability
Reference Values
Rotavirus Infections / metabolism*
Chemical
Reg. No./Substance:
0/Phospholipids; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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