| Effect of retinol on iron bioavailability from Iranian bread in a Caco-2 cell culture model. | |
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MedLine Citation:
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PMID: 16635564 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Iron and vitamin A deficiencies are among the most prevalent nutritional problems. There are no data on iron bioavailability in Iran. In addition, interaction of vitamin A with iron bioavailability is not well documented, so we determined iron bioavailability from the most widely consumed food in Iran, lavash bread, and the effect of vitamin A on this bioavailability. METHODS: In vivo human studies for determining iron bioavailability are cumbersome, time consuming, and costly to perform, so we used an in vitro model in Caco-2 cells. Bread samples were digested with or without vitamin A (10 microg/1.0 g of dried bread). We used an iron solution containing vitamin C as a positive control. Iron absorption was measured using 59FeCl3. Bioavailability was defined as the percentage of radiolabeled iron taken up and transferred by Caco-2 cells after 1.5 h of incubation. Experiments were carried out two to four times. RESULTS: Mean +/- standard deviations for iron bioavailability in bread samples digested without or with additional vitamin A and in controls were 2.53 +/- 1.55%, 6.62 +/- 3.40%, and 20.80 +/- 2.30%, respectively (P < 0.001). Vitamin A caused a 2.62-fold increase in iron absorption from bread samples. CONCLUSIONS: Iranian lavash bread has low iron bioavailability, but this can be increased by vitamin A supplementation. Increasing vitamin A intake can be considered as a method for increasing iron bioavailability, thus combating iron and vitamin A deficiencies simultaneously. |
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Authors:
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Bahram Pourghassem Gargari; Seyed Valee Razavieh; Soltanali Mahboob; Behrooz Niknafs; Hossein Kooshavar |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-04-25 |
Journal Detail:
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Title: Nutrition (Burbank, Los Angeles County, Calif.) Volume: 22 ISSN: 0899-9007 ISO Abbreviation: Nutrition Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-05-17 Completed Date: 2006-10-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8802712 Medline TA: Nutrition Country: United States |
Other Details:
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Languages: eng Pagination: 638-44 Citation Subset: IM |
Affiliation:
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Department of Nutrition and Biochemistry, Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. bahrampg@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biological Availability Bread* Caco-2 Cells Humans Iran Iron / deficiency Iron Radioisotopes Iron, Dietary / pharmacokinetics* Vitamin A / pharmacology* Vitamin A Deficiency / prevention & control Vitamins / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Iron Radioisotopes; 0/Iron, Dietary; 0/Vitamins; 11103-57-4/Vitamin A; 7439-89-6/Iron |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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