Document Detail


Effect of retinol on iron bioavailability from Iranian bread in a Caco-2 cell culture model.
MedLine Citation:
PMID:  16635564     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Iron and vitamin A deficiencies are among the most prevalent nutritional problems. There are no data on iron bioavailability in Iran. In addition, interaction of vitamin A with iron bioavailability is not well documented, so we determined iron bioavailability from the most widely consumed food in Iran, lavash bread, and the effect of vitamin A on this bioavailability. METHODS: In vivo human studies for determining iron bioavailability are cumbersome, time consuming, and costly to perform, so we used an in vitro model in Caco-2 cells. Bread samples were digested with or without vitamin A (10 microg/1.0 g of dried bread). We used an iron solution containing vitamin C as a positive control. Iron absorption was measured using 59FeCl3. Bioavailability was defined as the percentage of radiolabeled iron taken up and transferred by Caco-2 cells after 1.5 h of incubation. Experiments were carried out two to four times. RESULTS: Mean +/- standard deviations for iron bioavailability in bread samples digested without or with additional vitamin A and in controls were 2.53 +/- 1.55%, 6.62 +/- 3.40%, and 20.80 +/- 2.30%, respectively (P < 0.001). Vitamin A caused a 2.62-fold increase in iron absorption from bread samples. CONCLUSIONS: Iranian lavash bread has low iron bioavailability, but this can be increased by vitamin A supplementation. Increasing vitamin A intake can be considered as a method for increasing iron bioavailability, thus combating iron and vitamin A deficiencies simultaneously.
Authors:
Bahram Pourghassem Gargari; Seyed Valee Razavieh; Soltanali Mahboob; Behrooz Niknafs; Hossein Kooshavar
Related Documents :
17926834 - Deferiprone: second-line treatment of iron overload in the absence of a better alternat...
20019924 - Home fortification with micronutrient sprinkles - a new approach for the prevention and...
17305134 - Electrochemically fabricated zero-valent iron, iron-nickel, and iron-palladium nanowire...
17241724 - Halide salts accelerate degradation of high explosives by zerovalent iron.
12450914 - Inadequate nutrient intakes among homebound elderly and their correlation with individu...
10458884 - Changes in olfactory responsiveness by the domestic chick after early exposure to odora...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-04-25
Journal Detail:
Title:  Nutrition (Burbank, Los Angeles County, Calif.)     Volume:  22     ISSN:  0899-9007     ISO Abbreviation:  Nutrition     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-17     Completed Date:  2006-10-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8802712     Medline TA:  Nutrition     Country:  United States    
Other Details:
Languages:  eng     Pagination:  638-44     Citation Subset:  IM    
Affiliation:
Department of Nutrition and Biochemistry, Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. bahrampg@yahoo.com
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biological Availability
Bread*
Caco-2 Cells
Humans
Iran
Iron / deficiency
Iron Radioisotopes
Iron, Dietary / pharmacokinetics*
Vitamin A / pharmacology*
Vitamin A Deficiency / prevention & control
Vitamins / pharmacology*
Chemical
Reg. No./Substance:
0/Iron Radioisotopes; 0/Iron, Dietary; 0/Vitamins; 11103-57-4/Vitamin A; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Selective induction of inducible nitric oxide synthase in pancreatic islet of rat after an intraveno...
Next Document:  Body composition and resting energy expenditure in elderly male patients with chronic obstructive pu...