Document Detail

Effect of retinoic acid and phorbol-12-myristate-13-acetate on glycosyltransferase activities in normal and transformed cells.
MedLine Citation:
PMID:  3100023     Owner:  NLM     Status:  MEDLINE    
Retinoic acid was found to increase the activity of cytidine monophosphosialic acid:lactosylceramide sialyltransferase activity in a nontransformed clonal hamster cell line, NIL 8, and a virally transformed clone, NIL 8-HSV. The potent tumor promoter phorbol-12-myristate-13-acetate (PMA) had no significant effect on sialyltransferase activity in NIL 8 cells but stimulated this activity almost 6-fold when added to NIL 8-HSV cells. There was a synergistically additive effect on sialyltransferase activity when PMA was added to NIL 8 cells in concert with retinoic acid. On the other hand neither PMA nor retinoic acid had an appreciable effect on two other glycosyltransferases measured, uridine diphospho-N-acetylgalactosamine:globotriaosylceramide N-acetylgalactosaminyl-transferase and uridine diphosphogalactose:asialoagalactofetuin galactosyltransferase. Examination of sialyltransferase activity in a human epidermoid carcinoma cell line showed a large increase in enzyme activity in response to retinoic acid administration. Two nontransformed hamster cell lines had less basal sialyltransferase activity but also showed marked elevations after retinoic acid treatment. It is proposed that one of the molecular mechanisms underlying the biological effects of retinoic acid and PMA may be an increase in sialyltransferase activity. Possible regulatory mechanisms are discussed.
J R Moskal; M W Lockney; C C Marvel; J E Trosko; C C Sweeley
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  47     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1987 Feb 
Date Detail:
Created Date:  1987-03-04     Completed Date:  1987-03-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  787-90     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cell Transformation, Neoplastic*
Clone Cells
Galactosyltransferases / metabolism*
Retroviridae / genetics
Sialyltransferases / metabolism*
Tetradecanoylphorbol Acetate / pharmacology*
Tretinoin / pharmacology*
Grant Support
Reg. No./Substance:
16561-29-8/Tetradecanoylphorbol Acetate; 302-79-4/Tretinoin; EC 2.4.1.-/Galactosyltransferases; EC 2.4.99.-/Sialyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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