Document Detail

Effect of retinoic acid on differentiation of cultured pig preadipocytes.
MedLine Citation:
PMID:  9027555     Owner:  NLM     Status:  MEDLINE    
We studied the effect of retinoic acid on the differentiation of cultured porcine preadipocytes. Porcine preadipocytes were cultured in serum-free medium (DME/F12 medium containing 100 nM insulin, 10 micrograms/mL transferrin, and 50 ng/mL hydrocortisone). Addition of increasing amounts of retinoic acid (1 nM to 20 microM) to the medium reduced glycerol-3-phosphate dehydrogenase (GPDH) activity, a late marker of preadipocyte differentiation. At lower concentrations (0.1 to 10 nM), retinoic acid had no effect on the GPDH activity. Addition of retinoic acid (10 microM) for 24 h during the early stage of development (d 1) greatly inhibited the GPDH activity. After the cells were differentiated, however, retinoic acid no longer had any effect. Therefore, retinoic acid was most effective in undifferentiated cells. Following a 24-h exposure of porcine preadipocytes to retinoic acid at d 1, Northern blot analysis showed that there was a decrease in lipoprotein lipase and adipsin mRNA levels. The results suggest that retinoic acid is a potent inhibitor of porcine preadipocyte differentiation in primary culture.
A Suryawan; C Y Hu
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of animal science     Volume:  75     ISSN:  0021-8812     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-04-17     Completed Date:  1997-04-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  112-7     Citation Subset:  IM    
Department of Animal Sciences, Oregon State University, Corvallis 97331, USA.
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MeSH Terms
Adipocytes / cytology*,  metabolism,  physiology
Blotting, Northern
Cell Differentiation / drug effects,  physiology
Cells, Cultured
Complement Factor D
Culture Media / chemistry,  pharmacology
Dose-Response Relationship, Drug
Gene Expression Regulation
Glycerolphosphate Dehydrogenase / analysis,  metabolism
Hydrocortisone / analysis,  pharmacology
Insulin / analysis,  pharmacology
Lipoprotein Lipase / analysis,  genetics,  metabolism
RNA, Messenger / analysis,  genetics,  metabolism
Serine Endopeptidases / analysis,  genetics,  metabolism
Stem Cells / chemistry,  cytology*,  metabolism
Swine / metabolism,  physiology*
Transferrin / analysis,  pharmacology
Tretinoin / analysis,  pharmacology*
Triiodothyronine / analysis,  pharmacology
Grant Support
Reg. No./Substance:
0/Culture Media; 0/RNA, Messenger; 11061-68-0/Insulin; 11096-37-0/Transferrin; 302-79-4/Tretinoin; 50-23-7/Hydrocortisone; 6893-02-3/Triiodothyronine; EC 1.1.-/Glycerolphosphate Dehydrogenase; EC Lipase; EC 3.4.21.-/Serine Endopeptidases; EC Factor D

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