| Effect of retinoic acid on differentiation of cultured pig preadipocytes. | |
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MedLine Citation:
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PMID: 9027555 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We studied the effect of retinoic acid on the differentiation of cultured porcine preadipocytes. Porcine preadipocytes were cultured in serum-free medium (DME/F12 medium containing 100 nM insulin, 10 micrograms/mL transferrin, and 50 ng/mL hydrocortisone). Addition of increasing amounts of retinoic acid (1 nM to 20 microM) to the medium reduced glycerol-3-phosphate dehydrogenase (GPDH) activity, a late marker of preadipocyte differentiation. At lower concentrations (0.1 to 10 nM), retinoic acid had no effect on the GPDH activity. Addition of retinoic acid (10 microM) for 24 h during the early stage of development (d 1) greatly inhibited the GPDH activity. After the cells were differentiated, however, retinoic acid no longer had any effect. Therefore, retinoic acid was most effective in undifferentiated cells. Following a 24-h exposure of porcine preadipocytes to retinoic acid at d 1, Northern blot analysis showed that there was a decrease in lipoprotein lipase and adipsin mRNA levels. The results suggest that retinoic acid is a potent inhibitor of porcine preadipocyte differentiation in primary culture. |
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Authors:
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A Suryawan; C Y Hu |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of animal science Volume: 75 ISSN: 0021-8812 ISO Abbreviation: J. Anim. Sci. Publication Date: 1997 Jan |
Date Detail:
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Created Date: 1997-04-17 Completed Date: 1997-04-17 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8003002 Medline TA: J Anim Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 112-7 Citation Subset: IM |
Affiliation:
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Department of Animal Sciences, Oregon State University, Corvallis 97331, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes
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cytology*,
metabolism,
physiology Animals Blotting, Northern Cell Differentiation / drug effects, physiology Cells, Cultured Complement Factor D Culture Media / chemistry, pharmacology Dose-Response Relationship, Drug Gene Expression Regulation Glycerolphosphate Dehydrogenase / analysis, metabolism Hydrocortisone / analysis, pharmacology Insulin / analysis, pharmacology Lipoprotein Lipase / analysis, genetics, metabolism RNA, Messenger / analysis, genetics, metabolism Serine Endopeptidases / analysis, genetics, metabolism Stem Cells / chemistry, cytology*, metabolism Swine / metabolism, physiology* Transferrin / analysis, pharmacology Tretinoin / analysis, pharmacology* Triiodothyronine / analysis, pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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DK 42536/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/RNA, Messenger; 11061-68-0/Insulin; 11096-37-0/Transferrin; 302-79-4/Tretinoin; 50-23-7/Hydrocortisone; 6893-02-3/Triiodothyronine; EC 1.1.-/Glycerolphosphate Dehydrogenase; EC 3.1.1.34/Lipoprotein Lipase; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.46/Complement Factor D |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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