Document Detail

Effect of retinoic acid and 12-O-tetradecanoyl phorbol-13-acetate on the binding of epidermal growth factor to its cellular receptors.
MedLine Citation:
PMID:  6288114     Owner:  NLM     Status:  MEDLINE    
Binding of 125I-labelled epidermal growth factor (EGF) to C3H/2K cells and the effect of a tumor promotor, 12-O-tetradecanoyl phorbol-13-acetate (TPA) and of a tumor promotor antagonist, retinoic acid, on the binding was studied. Scatchard plot analysis of the binding showed the presence of two types of binding sites with different affinity to EGF. Treatment of the cells with retinoic acid for 1 h resulted in elevation of the affinity of both sites without changing their number per cell. Prolonged exposure to retinoic acid abrogated this elevation of the affinity and caused cycloheximide-sensitive increase of the number of the binding sites of both types. TPA inhibited binding of EGF to the cells by abolishing the binding to the high affinity sites, whereas retinoic acid, in the presence of TPA, enhanced it by increasing the number of the low affinity sites.
M Saito; I Ueno; K Egawa
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  717     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1982 Aug 
Date Detail:
Created Date:  1982-12-02     Completed Date:  1982-12-02     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  301-4     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
Epidermal Growth Factor / metabolism*
Mice, Inbred C3H
Phorbols / pharmacology*
Receptor, Epidermal Growth Factor
Receptors, Cell Surface / drug effects,  metabolism*
Structure-Activity Relationship
Tetradecanoylphorbol Acetate / pharmacology*
Tretinoin / pharmacology*
Vitamin A / pharmacology
Reg. No./Substance:
0/Phorbols; 0/Receptors, Cell Surface; 11103-57-4/Vitamin A; 16561-29-8/Tetradecanoylphorbol Acetate; 302-79-4/Tretinoin; 62229-50-9/Epidermal Growth Factor; EC, Epidermal Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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