Document Detail

Effect of resveratrol incorporated in liposomes on proliferation and UV-B protection of cells.
MedLine Citation:
PMID:  18718515     Owner:  NLM     Status:  MEDLINE    
The possibility of improving the efficacy of resveratrol, a polyphenol with strong antioxidant and free-radical scavenging properties, on cell proliferation and photoprotection by liposomal incorporation was investigated. Oligolamellar vesicles of different lipid compositions, loaded with resveratrol, were prepared and characterized by evaluating size, zeta potential, incorporation efficiency, electron microscopy and stability over 60 days. The effect of free and liposomal resveratrol on the viability of HEK 293 cells and their photoprotection after UV-B irradiation was assessed by the MTS method. Resveratrol decreased the cell viability at 100microM concentration, while at 10microM increased cell proliferation and also achieved the most effective photoprotection. Photomicrographs of the treated cells from inverted light and fluorescence microscopy demonstrated resveratrol effectiveness at 10microM, as well as its toxicity at higher concentrations, based on changes in cell shape, detachment and apoptotic features. Interestingly, liposomes prevented the cytotoxicity of resveratrol at high concentrations, even at 100microM, avoiding its immediate and massive intracellular distribution, and increased the ability of resveratrol to stimulate the proliferation of the cells and their ability to survive under stress conditions caused by UV-B light.
C Caddeo; K Teskac; C Sinico; J Kristl
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-31
Journal Detail:
Title:  International journal of pharmaceutics     Volume:  363     ISSN:  0378-5173     ISO Abbreviation:  Int J Pharm     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-09-22     Completed Date:  2008-12-12     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  7804127     Medline TA:  Int J Pharm     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  183-91     Citation Subset:  IM    
University of Ljubljana, Faculty of Pharmacy, Ljubljana, Slovenia.
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MeSH Terms
Cell Line
Cell Proliferation / drug effects*,  radiation effects
Cell Shape / drug effects
Cell Survival / drug effects
Dose-Response Relationship, Drug
Drug Compounding
Drug Stability
Epithelial Cells / drug effects*,  radiation effects
Lipids / chemistry*
Microscopy, Fluorescence
Particle Size
Stilbenes / chemistry,  pharmacology*,  toxicity
Sunscreening Agents / chemistry,  pharmacology*,  toxicity
Time Factors
Ultraviolet Rays*
Reg. No./Substance:
0/Lipids; 0/Liposomes; 0/Stilbenes; 0/Sunscreening Agents; Q369O8926L/resveratrol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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