| Effect of respiratory muscle training on GLUT-4 in the sheep diaphragm. | |
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MedLine Citation:
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PMID: 10949006 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Endurance exercise training is associated with enhanced glucose uptake and hence improvement in carbohydrate metabolism. Glucose transport (GLUT) membrane proteins are regulated by a variety of physiological stimuli, including exercise. In limb muscle, both acute exercise and endurance training enhance the expression of the skeletal muscle transporter, GLUT-4. The purpose of this study is to determine whether chronic loading enhances GLUT-4 expression in the diaphragm. METHODS: The effect of chronic inspiratory flow resistive (IFR) loading on diaphragm GLUT-4 was studied in a model of respiratory muscle endurance training in sheep. IFR loads (resistance 50-100 cm H2O x L(-1) x s(-1)) were maintained for 3 h x d(-1), 5-6 d x wk(-1) for 3 wk. Loading was adjusted so that PaO2 was >60 Torr and PaCO2 <45 Torr in room air. Six untrained sheep were used as controls. GLUT-4 protein and mRNA were analyzed by Western and Northern analysis respectively. RESULTS: GLUT-4 protein levels were two-fold greater in trained animals when compared with controls (P < 0.01). GLUT-4 mRNA levels in the trained muscles was not significantly different from controls. CONCLUSIONS: We conclude that in the sheep diaphragm, chronic IFR loading increases GLUT-4 protein levels. This increase may be one of the mechanisms contributing to the improved respiratory muscle endurance previously demonstrated in this animal model of respiratory muscle training. |
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Authors:
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A Bhandari; Y Xia; R Cortright; G L Dohm; A R Bazzy |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Medicine and science in sports and exercise Volume: 32 ISSN: 0195-9131 ISO Abbreviation: Med Sci Sports Exerc Publication Date: 2000 Aug |
Date Detail:
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Created Date: 2000-11-27 Completed Date: 2000-12-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8005433 Medline TA: Med Sci Sports Exerc Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1406-11 Citation Subset: IM; S |
Affiliation:
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Department of Pediatrics, Yale University School of Medicine, New Haven, CT 06510, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Northern Blotting, Western Breathing Exercises Diaphragm / metabolism*, physiology Glucose Transporter Type 4 Glyceraldehyde-3-Phosphate Dehydrogenases / genetics Monosaccharide Transport Proteins / genetics, metabolism* Muscle Proteins* RNA, Messenger / blood Sheep |
| Grant Support | |
ID/Acronym/Agency:
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HL-45128/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Glucose Transporter Type 4; 0/Monosaccharide Transport Proteins; 0/Muscle Proteins; 0/RNA, Messenger; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases |
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