Document Detail


Effect of repeated freeze-thaw cycles on urinary albumin-to-creatinine ratio.
MedLine Citation:
PMID:  19929272     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Urinary albumin-to-creatinine ratio (UACR) is widely used for diagnosis of chronic kidney disease in population investigation surveys. It is recommended that urinary albumin should be measured as soon as possible after urine is collected. It is not clear whether freezing and thawing affect the value of UACR and it is very inconvenient to measure UACR quickly in a population survey. The current study tries to determine the effect of repeated freezing and thawing on UACR to explore the possibility of freezing urine samples. METHODS: Fifty-three urine samples with abnormal urinary albumin (ranging from 22.9 mg/L to 891.9 mg/L) were selected. The albumin and creatinine were measured before freezing, then all sample were stored at -30 degrees C. After being thawed at room temperature, the albumin and creatinine concentrations were measured again. The samples were frozen and thawed for five times, and albumin and creatinine were measured after each thawing. The measurements of albumin, creatinine, and UACR after each thawing were calculated and compared with its initial values using multiple comparisons of one-way ANOVA. RESULTS: Compared with its initial value, urine albumin, creatinine and UACR all did not show any significant differences (p > 0.05). CONCLUSIONS: It is feasible to freeze urine samples for future measurement of UACR. Urine samples can be safely frozen and thawed at least five times.
Authors:
Yunfei Bao; Li Zuo
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Scandinavian journal of clinical and laboratory investigation     Volume:  69     ISSN:  1502-7686     ISO Abbreviation:  Scand. J. Clin. Lab. Invest.     Publication Date:  2009  
Date Detail:
Created Date:  2009-12-17     Completed Date:  2010-02-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404375     Medline TA:  Scand J Clin Lab Invest     Country:  England    
Other Details:
Languages:  eng     Pagination:  886-8     Citation Subset:  IM    
Affiliation:
Institute of Nephrology, Peking University First Hospital, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Albuminuria / urine*
Analysis of Variance
Creatinine / urine*
Freezing*
Humans
Chemical
Reg. No./Substance:
60-27-5/Creatinine

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