Document Detail

Effect of renin gene transfer on blood pressure in the spontaneously hypertensive rat.
MedLine Citation:
PMID:  9453331     Owner:  NLM     Status:  MEDLINE    
To investigate whether molecular variation in the renin gene contributes to the greater blood pressure of spontaneously hypertensive rats (SHR) versus normotensive Brown Norway (BN) rats, we measured blood pressure in an SHR progenitor strain and an SHR congenic strain that are genetically identical except at the renin gene and an associated segment of chromosome 13 transferred from the BN strain. Backcross breeding and molecular selection at the renin locus were used to create the SHR congenic strain (designated SHR.BN-Ren) that carries the renin gene transferred from the normotensive BN strain. We found that transfer of the renin gene from the BN strain onto the genetic background of the SHR did not decrease blood pressure in rats fed either a normal or high-salt diet. In fact, the systolic blood pressures of the SHR congenic rats tended to be slightly greater than the systolic blood pressures of the SHR progenitor rats. However, the congenic strain exhibited lower serum high-density lipoprotein cholesterol, and greater levels of total cholesterol, very-low-density lipoprotein, and intermediate-density lipoprotein cholesterol during administration of a high-fat, high-cholesterol diet. These findings demonstrate that (1) under the environmental circumstances of the current study, the greater blood pressure of SHR versus BN rats cannot be explained by strain differences in the renin gene and (2) a quantitative trait locus affecting lipid metabolism exists on chromosome 13 within the transferred chromosome segment. The SHR.BN-Ren congenic strain may provide a useful new animal model for studying the interaction between high blood pressure and dyslipidemia in cardiovascular disease.
E St Lezin; W Liu; N Wang; J M Wang; V Kren; V Zidek; M Zdobinska; D Krenova; A Bottger; B F van Zutphen; M Pravenec
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hypertension     Volume:  31     ISSN:  0194-911X     ISO Abbreviation:  Hypertension     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-02-20     Completed Date:  1998-02-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  373-7     Citation Subset:  IM    
Department of Laboratory Medicine, University of California, San Francisco 94120, USA.
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MeSH Terms
Blood Pressure / genetics*,  physiology
Cholesterol / blood
Chromosome Mapping*
Crosses, Genetic
Gene Transfer Techniques
Genetic Markers
Heart Rate / genetics
Hypertension / blood,  genetics*
Lipoproteins / blood
Quantitative Trait, Heritable
Rats, Inbred BN
Rats, Inbred SHR
Renin / biosynthesis*,  genetics*
Triglycerides / blood
Grant Support
Reg. No./Substance:
0/Genetic Markers; 0/Lipoproteins; 0/Triglycerides; 57-88-5/Cholesterol; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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