Document Detail


Effect of relA function on the replication of plasmid pBR322 in Escherichia coli.
MedLine Citation:
PMID:  2423847     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Replication of the plasmid pBR322, and the accumulation and life time of its primer transcript, RNAII, and replication inhibitor, RNAI, were measured in an isogenic relA+/relA pair of E. coli strains during exponential growth, or following amino acid starvation, or during treatment with chloramphenicol. (1) The synthesis rates of RNAI and RNAII decreased during inhibition of protein synthesis in either strain, i.e. their promoters are not under stringent control; (2) during amino acid starvation, RNAI and RNAII lifetimes increased in complex, rel-dependent patterns; (3) the changes in RNAI and RNAII synthesis and accumulation had no immediate effect on the rate of plasmid replication; (4) continued plasmid replication requires a protein which is synthesized during amino acid deprivation or treatment with low concentrations of chloramphenicol in relA+, but not in relA bacteria.
Authors:
S Lin-Chao; H Bremer
Related Documents :
14559907 - Unique mechanism of action of the thiourea drug isoxyl on mycobacterium tuberculosis.
3533937 - Hormonal regulation of lipogenic enzymes in chick embryo hepatocytes in culture. expres...
21631947 - Effects of canola and corn oil mimetic on jurkat cells.
16663367 - In vitro fatty acid synthesis and complex lipid metabolism in the cyanobacterium anabae...
1878107 - Marine biosurfactants, ii. production and characterization of an anionic trehalose tetr...
3437827 - Isolation of campylobacter pyloridis from human gastric mucosa and characterization of ...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  203     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-07-14     Completed Date:  1986-07-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  150-3     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics*
DNA Helicases*
DNA Replication*
DNA-Binding Proteins*
Escherichia coli / genetics*
Kinetics
Plasmids*
Proteins*
RNA / genetics*
RNA, Bacterial / genetics*
Trans-Activators*
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
GM 15412/GM/NIGMS NIH HHS; GM 25945/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/Proteins; 0/RNA I; 0/RNA primers; 0/RNA, Bacterial; 0/Trans-Activators; 0/replication initiator protein; 63231-63-0/RNA; EC 3.6.1.-/DNA Helicases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effect of the bacterial growth rate on replication control of plasmid pBR322 in Escherichia coli.
Next Document:  Cloning part of the region encoding biosynthetic enzymes for surface antigen (O-antigen) of Salmonel...