Document Detail

Effect of relA function on the replication of plasmid pBR322 in Escherichia coli.
MedLine Citation:
PMID:  2423847     Owner:  NLM     Status:  MEDLINE    
Replication of the plasmid pBR322, and the accumulation and life time of its primer transcript, RNAII, and replication inhibitor, RNAI, were measured in an isogenic relA+/relA pair of E. coli strains during exponential growth, or following amino acid starvation, or during treatment with chloramphenicol. (1) The synthesis rates of RNAI and RNAII decreased during inhibition of protein synthesis in either strain, i.e. their promoters are not under stringent control; (2) during amino acid starvation, RNAI and RNAII lifetimes increased in complex, rel-dependent patterns; (3) the changes in RNAI and RNAII synthesis and accumulation had no immediate effect on the rate of plasmid replication; (4) continued plasmid replication requires a protein which is synthesized during amino acid deprivation or treatment with low concentrations of chloramphenicol in relA+, but not in relA bacteria.
S Lin-Chao; H Bremer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  203     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-07-14     Completed Date:  1986-07-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  150-3     Citation Subset:  IM    
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MeSH Terms
Bacterial Proteins / genetics*
DNA Helicases*
DNA Replication*
DNA-Binding Proteins*
Escherichia coli / genetics*
RNA / genetics*
RNA, Bacterial / genetics*
Transcription, Genetic
Grant Support
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/Proteins; 0/RNA I; 0/RNA primers; 0/RNA, Bacterial; 0/Trans-Activators; 0/replication initiator protein; 63231-63-0/RNA; EC 3.6.1.-/DNA Helicases

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