Document Detail

Effect of reduced oocyte aging on the outcome of rescue intracytoplasmic sperm injection.
MedLine Citation:
PMID:  16580372     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate the results of a novel protocol that allows to rescue IVF unfertilized oocytes by intracytoplasmic sperm injection (ICSI). DESIGN: Prospective clinical trial. SETTING: Private reproductive medical center. PATIENT(S): Thirty patients undergoing IVF. INTERVENTION(S): Controlled ovarian stimulation (COS), conventional IVF, rescue ICSI, embryo culture, and embryo transfer. MAIN OUTCOME MEASURE(S): Identification of unfertilized IVF oocytes 6 hours after insemination and fertilization, and developmental rates of those oocytes after rescue microinjection, as well implantation and pregnancy rates (PR). RESULT(S): All oocytes (392) from 30 patients were inseminated with standard IVF 3 hours after ovum pick-up. Polar body (PB) status was checked at decumulation and rechecked 3 hours later. Eighty-two oocytes were fertilized after IVF alone and 184 nonactivated oocytes (failed fertilization) were rescue microinjected and 166 of them fertilized (20 patients). Cleavage stage on day 2 was significantly more advanced and embryo grade was higher after standard IVF fertilization than after rescue ICSI. Eight of the 30 embryos transferred were implanted in the IVF-only patients (27%) and 8 of 68 embryos in the rescue ICSI patients (12%). CONCLUSION(S): Rescue ICSI of unfertilized IVF oocytes 6 hours after insemination (9 hours after egg retrieval) can provide normal fertilization, embryo development, and pregnancy; however, corresponding outcome parameters tend to be impaired in comparison to the standard IVF fertilization results.
Zsolt P Nagy; Laura F Rienzi; Filippo M Ubaldi; Ermanno Greco; Joe B Massey; Hilton I Kort
Publication Detail:
Type:  Clinical Trial; Comparative Study; Journal Article    
Journal Detail:
Title:  Fertility and sterility     Volume:  85     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-03     Completed Date:  2006-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  901-6     Citation Subset:  IM    
Reproductive Biology Associates, Atlanta, Georgia 30342, USA.
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MeSH Terms
Cell Aging / physiology*
Oocytes / physiology*
Pregnancy Rate*
Prospective Studies
Sperm Count
Sperm Injections, Intracytoplasmic* / methods

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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