| Effect of recombinant galectin-1 on the growth of immortal rat chondrocyte on chitosan-coated PLGA scaffold. | |
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MedLine Citation:
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PMID: 19998464 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of galectin-1 (GAL1) on the growth of immortal rat chondrocyte (IRC) on chitosan-modified PLGA scaffold is investigated. The experimental results showed that water absorption ratio of chitosan-modified PLGA scaffold was 70% higher than that of PLGA alone after immersion in ddH(2)O for 2 weeks, indicating that chitosan-modification significantly enhances the hydrophilicity of PLGA. The experimental results also showed that GALl efficiently and spontaneously coats the chitosan-PLGA scaffold surface to promote adhesion and growth of immortal rat chondrocyte (IRC). To investigate the effect of endogenous GAL1, the full-length GAL1 cDNAs were cloned and constructed into pcDNA3.1 vectors to generate a plasmid expressed in IRC (IRC-GAL1). The results showed that IRC-GAL1 growth was significantly higher than that of IRC on chitosan-PLGA scaffold. The GAL1-potentiated IRC growth on chitosan-PLGA scaffold was dose-dependently inhibited by TDG (specific inhibitor of GAL1 binding). These results strongly suggest that GAL1 is critical for enhancing IRC cell adhesion and growth on chitosan-PLGA scaffold. Moreover, GAL1-coating or expression tends to promote IRC cell-cell aggregation on chitosan-PLGA scaffold and significantly enhances IRC migration. These results suggest that GAL1 probably could induce tissue differentiation and facilitates cartilage reconstruction. In conclusion, the experimental results suggest that both GAL1 and chitosan are important for enhancing IRC cell adhesion and growth on PLGA scaffold, and GAL1 is a potential biomaterial for tissue engineering. |
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Authors:
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Shiang-Jiuun Chen; Chien-Chung Lin; Wei-Cheh Tuan; Ching-Shiow Tseng; Rong-Nan Huang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical materials research. Part A Volume: 93 ISSN: 1552-4965 ISO Abbreviation: J Biomed Mater Res A Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-04-27 Completed Date: 2010-12-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: United States |
Other Details:
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Languages: eng Pagination: 1482-92 Citation Subset: IM |
Copyright Information:
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(c) 2009 Wiley Periodicals, Inc. |
Affiliation:
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Department of Life Science, Institute of Ecology and Evolutionary Biology and TechComm-5, College of Life Science, National Taiwan University, Taipei 106, Taiwan, Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cartilage / pathology Cell Adhesion Cell Aggregation Cell Movement Chitosan / chemistry* Chondrocytes / cytology* Galectin 1 / chemistry* Lactic Acid / chemistry* Polyglycolic Acid / chemistry* Rats Recombinant Proteins / chemistry Thiogalactosides / chemistry Tissue Engineering / instrumentation*, methods Tissue Scaffolds / chemistry* Water / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Galectin 1; 0/Recombinant Proteins; 0/Thiogalactosides; 0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid; 7732-18-5/Water; 80441-61-8/thiodigalactoside; 9012-76-4/Chitosan |
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