| Effect of recombinant Panton-Valentine leukocidin in vitro on apoptosis and cytokine production of human alveolar macrophages. | |
| | |
MedLine Citation:
|
PMID: 20453909 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Panton-Valentine leukocidin (PVL) is associated with rare cases of necrotizing pneumonia that occur in otherwise healthy individuals. Human alveolar macrophages (HAMs) are major effector cells in host defense against infections. However, the impact of PVL on HAMs is uncertain. We evaluated the role of PVL in cytotoxicity and production of inflammatory cytokines secreted by HAMs. HAMs were purified from bronchoalveolar lavage fluid. Recombinant PVL (rPVL) was used in the study to interfere with HAM apoptosis and cytokine production in vitro. Hoechst 33342 fluorescence staining, transmission electron microscopy examination, and flow cytometry indicated that rPVL (10 nmol/L) treatment resulted in HAMs with markedly apoptotic characteristics, and HAMs treated with rPVL at 100 nmol/L showed clear indication of necrosis. A treatment of rPVL at 10 nmol/L elicited the secretion of IL-10 by HAMs relative to untreated control cells, but there was a slight decrease in the constitutive secretion of tumor necrosis factor (TNF)-alpha. Our results indicate that PVL-treated samples decreased HAM viability, leading to apoptosis at low concentrations and necrosis at high concentrations. In addition, PVL-treated cells released increased amounts of IL-10 and decreased amounts of TNF-alpha under apoptosis-inducing concentrations. Therefore, we speculated that PVL could play a negative role in HAM function at lower concentrations. |
| | |
Authors:
|
Benquan Wu; Wenxian Zhang; Jing Huang; Hui Liu; Tiantuo Zhang |
Related Documents
:
|
6240819 - Characterization of the inflammatory cells in progressing tumor-like alveolar hydatid c... 12042809 - Cd1d-dependent macrophage-mediated clearance of pseudomonas aeruginosa from lung. 7297519 - Dose response of the pulmonary macrophagic system to various particulates and its relat... 2200279 - Clearance and recycling of pulmonary surfactant. 6338779 - Lung inflammation: role of endogenous chemotactic factors in attracting polymorphonucle... 937839 - Cellular and humoral bronchopulmonary immune response of rabbits immunized with thermop... 11988579 - Contact-dependent demyelination by mycobacterium leprae in the absence of immune cells. 18826479 - Current concepts of molecular defence mechanisms operative during urinary tract infection. 22324889 - Explants of intact endometrium to model bovine innate immunity and inflammation ex vivo. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Canadian journal of microbiology Volume: 56 ISSN: 1480-3275 ISO Abbreviation: Can. J. Microbiol. Publication Date: 2010 Mar |
Date Detail:
|
Created Date: 2010-05-10 Completed Date: 2010-07-12 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372707 Medline TA: Can J Microbiol Country: Canada |
Other Details:
|
Languages: eng Pagination: 229-35 Citation Subset: IM |
Affiliation:
|
Department of Internal Medicine, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, P R China. zswbq@163.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects* Bacterial Toxins / genetics, toxicity* Benzimidazoles / metabolism Bronchoalveolar Lavage Fluid / cytology Cells, Cultured Exotoxins / genetics, toxicity* Flow Cytometry Gene Expression Regulation / drug effects Humans Interleukin-10 / metabolism*, secretion Leukocidins / genetics, toxicity* Macrophages, Alveolar / drug effects*, metabolism, ultrastructure Microscopy, Electron, Transmission Necrosis Recombinant Proteins / genetics, toxicity* Tumor Necrosis Factor-alpha / metabolism*, secretion |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Toxins; 0/Benzimidazoles; 0/Exotoxins; 0/Leukocidins; 0/Panton-Valentine leukocidin; 0/Recombinant Proteins; 0/Tumor Necrosis Factor-alpha; 130068-27-8/Interleukin-10; 23491-52-3/HOE 33342 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Validation of a partial rpoB gene sequence as a tool for phylogenetic identification of aeromonads i...
Next Document: Characterization of arsenic-resistant bacteria from the rhizosphere of arsenic hyperaccumulator Pter...