Document Detail

Effect of reactive cell density on net [2-14C]acetate uptake into rat brain: labeling of clusters containing GFAP+- and lectin+-immunoreactive cells.
MedLine Citation:
PMID:  12510019     Owner:  NLM     Status:  MEDLINE    
Astrocytic proliferation is a hallmark of brain injury, but the biological functions and metabolic activities of reactive astrocytes in vivo are poorly understood. [2-14C]Acetate, which is preferentially transported into and, therefore, metabolized by astrocytes, was used to assess injury- and trophic factor-induced changes in astrocyte metabolic activity. Local rates of net [2-14C]acetate uptake and glucose utilization (CMR(glc)), determined with [14C]deoxyglucose to assay overall metabolic activity of all brain cells, were assayed 7 days after a cannula placement; adjacent brain sections were immunostained to identify glial fibrillary acidic protein-positive (GFAP(+)) astrocytes and microglia plus macrophages (lectin-positive cells). GFAP(+) cells were abundant in tissue surrounding the cannula compared to the contralateral hemisphere, whereas lectin(+) cells were restricted to the wound boundary. CMR(glc) fell 25% in regions enriched in reactive astrocytes compared to the homologous contralateral hemisphere, whereas [14C]acetate uptake increased slightly (6%) but statistically significantly; metabolism of both tracers in 13 other brain structures was unchanged. Injection of basic fibroblast growth factor (b-FGF) into cerebral cortex or superior colliculus produced fiber-rich cell clusters containing both GFAP(+) and lectin(+) cells that had a 37% increase in [14C]acetate uptake; GFAP(+)-cell density rose in the nearby neuropil but the corresponding change in [14C]acetate uptake was small (6-8%). Sensory stimulation did not alter [14C]acetate uptake into the clusters. Thus, [14C]acetate uptake was relatively stable with respect to changes in the density of reactive astrocytes that are dispersed throughout the neuropil and to changes in cellular activity arising from sensory stimulation. In contrast, b-FGF-induced cell clusters that contain mixed cell types and numerous fibers accumulated higher levels of [14C]acetate, raising the possibility that increased uptake might be due to high numbers of activated astrocytes and, perhaps, acetate metabolism by other cell types.
Neslihan Cetin; Kelly Ball; Murat Gokden; Nancy F Cruz; Gerald A Dienel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurochemistry international     Volume:  42     ISSN:  0197-0186     ISO Abbreviation:  Neurochem. Int.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-01-02     Completed Date:  2003-04-09     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8006959     Medline TA:  Neurochem Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  359-74     Citation Subset:  IM    
Department of Neurology, University of Arkansas for Medical Sciences, Slot 500 4301 W. Markham St., Little Rock 72205, USA.
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MeSH Terms
Acetates / metabolism*
Antimetabolites / metabolism
Astrocytes / metabolism
Brain / cytology*,  drug effects,  metabolism*
Cell Count
Coloring Agents
Deoxyglucose / metabolism
Endothelium / cytology
Fibroblast Growth Factor 2 / pharmacology
Foreign Bodies / metabolism,  pathology
Glial Fibrillary Acidic Protein / metabolism*
Lectins / metabolism
Macrophages / drug effects,  metabolism
Microglia / drug effects,  metabolism
Rats, Wistar
Superior Colliculi / physiology
Grant Support
Reg. No./Substance:
0/Acetates; 0/Antimetabolites; 0/Coloring Agents; 0/Glial Fibrillary Acidic Protein; 0/Lectins; 103107-01-3/Fibroblast Growth Factor 2; 154-17-6/Deoxyglucose

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