| Effect of reaction temperature on the determination of rat serum IgE by the avidin-biotin method. | |
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MedLine Citation:
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PMID: 8987869 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We established an avidin-biotin method for the sensitive determination of rat IgE and found that a non-specific signal was generated depending on the reaction temperature. When the sera of rats immunized or not with ovalbumin (OVA) were fractionated in a hydroxyapatite column and OVA-specific IgE was determined by the avidin-biotin method at 4 degrees C or 37 degrees C, OVA-specific IgE peaks were detected at 37 degrees C, even with nonimmunized rats, but not at 4 degrees C. |
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Authors:
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K Yamada; A Nakao; M Takasugi; Y Itoh; N A Hassan; M Sugano |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 60 ISSN: 0916-8451 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 1996 Nov |
Date Detail:
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Created Date: 1997-02-24 Completed Date: 1997-02-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 1891-2 Citation Subset: B |
Affiliation:
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Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Avidin / chemistry Biotin / chemistry Enzyme-Linked Immunosorbent Assay Hydroxyapatites Immunoglobulin E / analysis*, isolation & purification Indicators and Reagents Male Ovalbumin / immunology Rats Rats, Inbred BN Temperature |
| Chemical | |
Reg. No./Substance:
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0/Hydroxyapatites; 0/Indicators and Reagents; 1405-69-2/Avidin; 37341-29-0/Immunoglobulin E; 58-85-5/Biotin; 9006-59-1/Ovalbumin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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