| Effect of pyruvate on polyol pathway and lens epithelial cells apoptosis in diabetic rats. | |
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MedLine Citation:
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PMID: 17378160 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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PURPOSE: To investigate the effect of polyol pathway on lens epithelial cells apoptosis and the activity of caspase-3 and its reversal by pyruvate in diabetic rats. METHODS: 220 Wister rats were divided into 3 groups: control group, model group and treatment group. After streptozotocin (STZ) induced cataract, the treatment group received 2% pyruvate in the diet and drinking. The opacification of lens was detected by microscope every 2 weeks. On 4W, 8W and 12W of the experiment, glucose and sorbitol in the lens were quantified by high-performance liquid chromatography. The percentage of lens epithelial cells undergoing apoptosis was measured by Annexin V/PI staining. The activity of caspase-3 was analyzed by Western-blot. RESULTS: Studies show that there was significant increase of glucose, sorbitol in lens of model group, the apoptosis rate and caspase-3 activity of lens epithelial cells were also gradually increase. Pyruvate treatment decreased the levels of sorbitol, glucose, lens epithelial cells apoptosis and caspase-3 activity. The progress of cataract was also significantly delayed. CONCLUSIONS: Polyol pathway, possibly through regulation of the activity of caspase-3, can induce apoptosis of lens epithelial cell. Pyruvate ingested orally can effective inhibit diabetic cataractogenesis in rats through inhibit polyol pathway. |
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Authors:
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Yanxiu Qi; Jisong Zhang |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu Volume: 22 ISSN: 1000-4432 ISO Abbreviation: Yan Ke Xue Bao Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2007-03-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8605666 Medline TA: Yan Ke Xue Bao Country: China |
Other Details:
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Languages: eng Pagination: 259-64 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, The First Affiliated Hospital, China Medical University, Shenyang 110001, China. mengqi99@sina.com |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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