| Effect of pulsed-light treatment on milk proteins and lipids. | |
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MedLine Citation:
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PMID: 18290615 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pulsed-light treatment offers the food industry a new technology for food preservation. It allows the inactivation of numerous micro-organisms including most infectious foodborne pathogens. In addition to microbial destruction, one can also question whether pulsed-light treatment induced conformational changes in food components. To investigate this question, the influence of pulsed-light treatment on protein components of milk was evaluated by using UV spectroscopy, spectrofluorometry, electrophoresis, and determination of amino acid composition. Pulsed-light treatment resulted in an increase of UV absorbance at 280 nm. The intrinsic tryptophan fluorescence of beta-lactoglobulin (BLG) showed a 7 nm red shift after 10 pulses. SDS-PAGE showed the formation of dimers after treatment of BLG by 5 pulses and more. No significant changes in the amino acid composition of proteins and lipid oxidation were observed after pulsed-light treatment. The obtained results indicated changes in the polarity of the tryptophanyl residue microenvironment of BLG solutions or changes in the tryptophan indole structure and some aggregation of studied proteins. Hence, pulsed-light treatment did not lead to very significant changes in protein components; consequently, it could be applied to process protein foods for their better preservation. |
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Authors:
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Noura Elmnasser; Michèle Dalgalarrondo; Nicole Orange; Amina Bakhrouf; Thomas Haertlé; Michel Federighi; Jean-Marc Chobert |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-02-22 |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 56 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-03-20 Completed Date: 2008-06-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1984-91 Citation Subset: IM |
Affiliation:
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UMR-INRA 1014 SECALIM ENVN/ENITIAA, Nantes, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Animals Electrophoresis, Polyacrylamide Gel Fats / analysis* Food Preservation / methods* Lactoglobulins / analysis Light* / adverse effects Milk / chemistry*, radiation effects Milk Proteins / analysis* Spectrometry, Fluorescence Spectrophotometry, Ultraviolet |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Fats; 0/Lactoglobulins; 0/Milk Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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