Document Detail

Effect of protamine-2 deficiency on ICSI outcome.
MedLine Citation:
PMID:  15670415     Owner:  NLM     Status:  MEDLINE    
During spermiogenesis, histones are replaced by protamines (P1 and P2), resulting in sperm chromatin condensation followed by a halt to gene expression in haploid spermatids and spermatozoa. As a consequence, protamine deficiency and aberrant P1/P2 ratio have a profound effect on both fertilization and embryo development. However, reports on the effect of the P1/P2 ratio on fertilization and embryo development after intracytoplasmic sperm injection (ICSI) are contradictory between human and animal studies. The question that still remains to be elucidated is which type of protamine deficiency is most common among protamine deficient samples. The present study has a direct bearing on this issue investigating the correlation of the P1/P2 ratio with protamine deficiency, fertilization, embryo quality and embryo development in ICSI patients. This study was carried out on 71 patients. Chromomycin A3 (CMA3) staining was used to determine protamine deficiency. Since this procedure does not indicate the type of protamine deficiency, the P1/P2 ratio was evaluated by nuclear protein extraction, acetic acid urea polyacrylamide gel electrophoresis and analysis of protein bands with software. Polyclonal anti-P1 and anti-P2 antibodies were used to confirm P1 and P2 presence. Results show a negative significant correlation of fertilization rate with protamine deficiency and P1/P2 ratio. No significant correlation was observed between protamine deficiency and P1/P2 ratio. Therefore, it can be concluded that altered P1/P2 ratio effects fertilization rate and embryo quality which subsequently may affect implantation and pregnancy outcome.
M H Nasr-Esfahani; M Salehi; S Razavi; M Mardani; H Bahramian; K Steger; F Oreizi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  9     ISSN:  1472-6483     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2005-01-26     Completed Date:  2005-03-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  652-8     Citation Subset:  IM    
Royan Institute, Tehran, Iran.
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MeSH Terms
Chromomycin A3 / metabolism
Embryonic Development
Metabolism, Inborn Errors / physiopathology*
Protamines / metabolism*
Sperm Injections, Intracytoplasmic*
Spermatozoa / metabolism*
Staining and Labeling
Treatment Outcome
Reg. No./Substance:
0/PRM1 protein, human; 0/Protamines; 0/protamine 2; 7059-24-7/Chromomycin A3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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