Document Detail

Effect of prolonged intermittent hypoxia and exercise training on glucose tolerance and muscle GLUT4 protein expression in rats.
MedLine Citation:
PMID:  15591781     Owner:  NLM     Status:  MEDLINE    
We compared the chronic effect of intermittent hypoxia and endurance training on the glucose tolerance and GLUT4 protein expression in rat skeletal muscle. Thirty-two Sprague-Dawley rats were matched for weight and assigned to one of the following four groups: control, endurance training, hypoxia, or hypoxia followed by endurance training. Hypoxic treatment consisted of breathing 14% O2 for 12 h/day under normobaric conditions, and the training protocol consisted of making animals swim 2 times for 3 h/day. At the end of the 3rd week, an oral glucose tolerance test (OGTT) was performed 16 h after treatments. At the end of the 4th week, GLUT4 protein, mRNA, and glycogen storage in skeletal muscle were determined. Endurance training significantly improved OGTT results. Glycogen content and GLUT4 protein expression in the plantaris and red gastrocnemius, but not in the soleus or white gastrocnemius muscles, were also elevated. Chronic intermittent hypoxia also improved OGTT results, but did not alter GLUT4 protein expression. Additionally, hypoxia followed by exercise training produced significant increases in GLUT4 protein and mRNA in a greater number of muscles compared to endurance training alone. Both exercise training and hypoxia significantly reduced body mass, and an additive effect of both treatments was found. In conclusion, chronic intermittent hypoxia improved glucose tolerance in the absence of increased GLUT4 protein expression. This treatment facilitated the exercise training effect on muscle GLUT4 expression and glycogen storage. These new findings open the possibility of utilizing intermittent hypoxia, with or without exercise training, for the prevention and clinical treatment of type 2 diabetes or insulin resistance.
Li-Ling Chiu; Shih-Wei Chou; Yu-Min Cho; Hsin-Yi Ho; John L Ivy; Desmond Hunt; Paulus S Wang; Chia-Hua Kuo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical science     Volume:  11     ISSN:  1021-7770     ISO Abbreviation:  J. Biomed. Sci.     Publication Date:    2004 Nov-Dec
Date Detail:
Created Date:  2004-12-13     Completed Date:  2005-05-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421567     Medline TA:  J Biomed Sci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  838-46     Citation Subset:  IM    
Copyright Information:
2004 National Science Council, ROC and S. Karger AG, Basel
Laboratory of Exercise Biochemistry, Taipei Physical Education College, Taipei, Taiwan, ROC.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Analysis of Variance
Blotting, Northern
Blotting, Western
Body Weight
Glucose Tolerance Test
Glucose Transporter Type 4
Glycogen / metabolism
Insulin / metabolism
Insulin Resistance
Monosaccharide Transport Proteins / biosynthesis*
Muscle Proteins / biosynthesis*
Muscle, Skeletal / metabolism*
Muscles / metabolism*
Physical Conditioning, Animal*
RNA, Messenger / metabolism
Rats, Sprague-Dawley
Time Factors
Reg. No./Substance:
0/Glucose Transporter Type 4; 0/Monosaccharide Transport Proteins; 0/Muscle Proteins; 0/RNA, Messenger; 0/Slc2a4 protein, rat; 11061-68-0/Insulin; 9005-79-2/Glycogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effect of hypercholesterolemia on myocardial function in New Zealand white rabbits.
Next Document:  Hypoxia modulates expression of the 70-kD heat shock protein and reduces Leishmania infection in mac...