Document Detail

Effect of progesterone on the expression of bax and bcl-2 and on caspase activity in bovine luteal cells.
MedLine Citation:
PMID:  16303606     Owner:  NLM     Status:  MEDLINE    
Bovine luteal cells from days 6-10 and 11-15 of the estrous cycle were exposed (6 h) to factors that support or disrupt steroidogenesis. The expression of bcl-2 and bax and level of active caspase-3 in cells was measured. Progesterone (P4) increased (P<0.01) while staurosporine decreased (P<0.01-P<0.001) bcl-2 expression at both stages of the estrous cycle studied. In cells from 11-15 days of the estrous cycle expression of bcl-2 was stimulated (P<0.05) by prostaglandin (PG)E2 and inhibited (P<0.01) by 3,3',4,4'-tertrachlorobiphenyl (PCB)-77. Treatment with aminoglutethimide (blocker of cytochrome P450scc; 1.5 x 10(-4)M), nitric oxide donor (spermine NONOate), and staurosporine increased bax expression in cells collected from both experimental periods. The influence of these factors was greater in cells from days 11-15 (P<0.001) than by cells on days 6-10 (P<0.05) of the estrous cycle. PCB-77 stimulated expression of bax in cells from 11-15 days of cycle (P<0.01) only. Treatment of luteal cells with P4 and PGE2 for 24 h decreased (P<0.05) level of active caspase-3 while aminoglutethimide (P<0.05), spermine NONOate (P<0.05), and staurosporine (P<0.001) increased caspase-3 activity in the cells. Moreover, P4 decreased (P<0.05) while staurosporine increased (P<0.01) the ratio of bax/bcl-2 at both stages of the cycle. Aminoglutethimide, spermine NONOate and PCB increased (0<0.05) this ratio in cells on days 11-15 of the cycle. These results suggest that P4 concentrations in luteal cells protects against apoptosis, while disruption of steroidogenesis and reduced ability of luteal cells to produce P4 can induce cell death.
E Liszewska; R Rekawiecki; J Kotwica
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-06-09
Journal Detail:
Title:  Prostaglandins & other lipid mediators     Volume:  78     ISSN:  1098-8823     ISO Abbreviation:  Prostaglandins Other Lipid Mediat.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-11-23     Completed Date:  2006-04-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9808648     Medline TA:  Prostaglandins Other Lipid Mediat     Country:  United States    
Other Details:
Languages:  eng     Pagination:  67-81     Citation Subset:  IM    
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, P.O. Box 55, 10-718 Olsztyn, Poland.
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MeSH Terms
Aminoglutethimide / pharmacology
Base Sequence
Caspases / metabolism*
Corpus Luteum / drug effects*,  enzymology,  metabolism
DNA Primers
Enzyme Inhibitors / pharmacology
Progesterone / pharmacology*
Protein Kinase C / antagonists & inhibitors
Proto-Oncogene Proteins c-bcl-2 / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Staurosporine / pharmacology
bcl-2-Associated X Protein / metabolism*
Reg. No./Substance:
0/DNA Primers; 0/Enzyme Inhibitors; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 125-84-8/Aminoglutethimide; 57-83-0/Progesterone; 62996-74-1/Staurosporine; EC Kinase C; EC 3.4.22.-/Caspases

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