| Effect of pressure on the self-association of melittin. | |
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MedLine Citation:
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PMID: 6466646 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of increased hydrostatic pressure (1 bar to 1.8 kbar) on the self-association of melittin was measured by using the fluorescence anisotropy of its single tryptophan residue. The degree of self-association was found to decrease with increasing pressure. The volume change (delta V) for dissociation is surprisingly large. At low pressures, delta V for dissociation is near -150 mL/mol. The magnitude of the volume change decreased with increasing pressure, possibly as a result of pressure-induced compression of free volume trapped at the subunit interface region of the tetramer. Overall, the pressure-dependent association of melittin is comparable to that expected for hydrophobic interactions and to that found for micelle formation by detergents. |
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Authors:
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R B Thompson; J R Lakowicz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemistry Volume: 23 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1984 Jul |
Date Detail:
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Created Date: 1984-10-12 Completed Date: 1984-10-12 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3411-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bee Venoms
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metabolism* Fluorescence Polarization Macromolecular Substances Mathematics Melitten / metabolism* Pressure Protein Conformation Thermodynamics Tryptophan |
| Grant Support | |
ID/Acronym/Agency:
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GM 29318/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bee Venoms; 0/Macromolecular Substances; 20449-79-0/Melitten; 73-22-3/Tryptophan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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