Document Detail

Effect of pressure on the self-association of melittin.
MedLine Citation:
PMID:  6466646     Owner:  NLM     Status:  MEDLINE    
The effect of increased hydrostatic pressure (1 bar to 1.8 kbar) on the self-association of melittin was measured by using the fluorescence anisotropy of its single tryptophan residue. The degree of self-association was found to decrease with increasing pressure. The volume change (delta V) for dissociation is surprisingly large. At low pressures, delta V for dissociation is near -150 mL/mol. The magnitude of the volume change decreased with increasing pressure, possibly as a result of pressure-induced compression of free volume trapped at the subunit interface region of the tetramer. Overall, the pressure-dependent association of melittin is comparable to that expected for hydrophobic interactions and to that found for micelle formation by detergents.
R B Thompson; J R Lakowicz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  23     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1984 Jul 
Date Detail:
Created Date:  1984-10-12     Completed Date:  1984-10-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3411-7     Citation Subset:  IM    
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MeSH Terms
Bee Venoms / metabolism*
Fluorescence Polarization
Macromolecular Substances
Melitten / metabolism*
Protein Conformation
Grant Support
Reg. No./Substance:
0/Bee Venoms; 0/Macromolecular Substances; 20449-79-0/Melitten; 73-22-3/Tryptophan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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