Document Detail

Effect of pressure on pulse radiolysis reduction of proteins.
MedLine Citation:
PMID:  15953680     Owner:  NLM     Status:  MEDLINE    
Pulse radiolysis experiments were performed on proteins under pressure. Whereas many spectroscopic techniques have shown protein modifications at different pressure ranges, the present measurements performed using the water radiolysis allowed to generate radical species and to study the mechanisms implied in their reactions with proteins. This work gives the first results obtained on the effects of pressure on the rate constants of the proteins reduction by the hydrated electron at pressures up to 100 MPa. The reaction with the hydrated electron was investigated on two classes of protein: the horse myoglobin and the mussel metallothioneins. We have successively studied the influence of the pH value of metmyoglobin solutions (pH 6, 7 and 8) and the influence of the metals nature (Zn,Cu,Cd) bound to metallothioneins. For both protein, whatever the experimental conditions, the pressure does not influence the value of the reduction rate constant in the investigated range (0.1-100 MPa).
Céline Bataille; Gérard Baldacchino; Richard P Cosson; Michèle Coppo; Catherine Trehen; Georges Vigneron; Jean Philippe Renault; Serge Pin
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1724     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-08-05     Completed Date:  2005-09-15     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  432-9     Citation Subset:  IM    
Laboratoire Claude Fréjacques (URA 331 CEA/CNRS), DSM/DRECAM/Service de Chimie Moléculaire, CEA Saclay, 91191-Gif sur Yvette cedex, France.
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MeSH Terms
Hydroxyl Radical / chemistry,  metabolism
Metallothionein / chemistry*,  metabolism
Myoglobin / chemistry*,  metabolism
Pulse Radiolysis / instrumentation
Superoxides / chemistry,  metabolism
Reg. No./Substance:
0/Myoglobin; 11062-77-4/Superoxides; 3352-57-6/Hydroxyl Radical; 9038-94-2/Metallothionein

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