Document Detail

Effect of preservatives on ciliary beat frequency in human nasal epithelial cell culture: single versus multiple exposure.
MedLine Citation:
PMID:  17324538     Owner:  NLM     Status:  MEDLINE    
As preservatives may impair mucociliary clearance, we wanted to systematically study their time-dependent effect on the ciliary beat frequency (CBF) in human nasal epithelial cells (HNEC). CBF was determined using a high-speed digital imaging method. Five preservatives were selected including benzalkonium chloride, phenylethyl alcohol, methylparaben, propylparaben and chlorbutol. We were interested in the effect of these preservatives on CBF after single and repetitive exposure. Methylparaben (0.0033%), propylparaben (0.0017%) and chlorbutol (0.005%) did not impair CBF, neither after a single short-term exposure period, nor after a single long-term exposure period. Long-term exposure to benzalkonium chloride (0.001%), phenylethyl alcohol (0.125%) and a combination of methyl- and propylparaben (0.0033 and 0.0017%) significantly decreased CBF. After a short-term exposure period, CBF recovered for phenylethyl alcohol and the combination of methyl- and propylparaben. Benzalkonium chloride decreased CBF non-reversibly. For two compounds, the effect on CBF was evaluated after repetitive exposure during 15min for 5 consecutive days. Benzalkonium chloride resulted in ciliostasis for all concentrations tested after 5 days. Phenylethyl alcohol revealed a concentration-dependent effect on CBF, but no ciliostasis was observed. In conclusion, methylparaben, propylparaben and chlorbutol can be considered as cilio-friendly. Repetitive exposure revealed a cumulative effect on CBF for benzalkonium chloride and phenylethyl alcohol.
R Mallants; M Jorissen; P Augustijns
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-21
Journal Detail:
Title:  International journal of pharmaceutics     Volume:  338     ISSN:  0378-5173     ISO Abbreviation:  Int J Pharm     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-28     Completed Date:  2007-07-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7804127     Medline TA:  Int J Pharm     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  64-9     Citation Subset:  IM    
Laboratory for Pharmacotechnology and Biopharmacy, Katholieke Universiteit Leuven, Leuven, Belgium.
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MeSH Terms
Cells, Cultured
Dose-Response Relationship, Drug
Epithelial Cells / drug effects,  metabolism
Mucociliary Clearance / drug effects*
Nasal Mucosa / cytology,  drug effects*
Preservatives, Pharmaceutical / pharmacology*
Reg. No./Substance:
0/Preservatives, Pharmaceutical

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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