Document Detail

Effect of pregnancy-specific protein B on luteal cell progesterone, prostaglandin, and oxytocin production during two stages of the bovine estrous cycle.
MedLine Citation:
PMID:  8582856     Owner:  NLM     Status:  MEDLINE    
This experiment was designed to evaluate the effects of pregnancy-specific protein B (PSPB) on luteal cell progesterone (P4), PGF2 alpha, PGE2, and oxytocin secretion. Corpora lutea were collected during the mid (d 10 to 12; n = 5) or late luteal (d 17 to 18; n = 5) stage of the estrous cycle. Large and small cells (1.5 x 10(5)/well) were treated with PSPB (0, 2.5, or 5.0 micrograms) and LH (0, 50, or 100 ng) in a 3 x 3 factorial arrangement. Cells were incubated for 18 h before adding treatments; after treatments, medium was collected at 6 and 12 h. During the 18-h pretreatment period, P4, PGF2 alpha, PGE2, and oxytocin production was similar between the prospective treatment groups. The PSPB did not affect P4 production. Stage of the cycle (stage) x time interaction (P < .001) indicated that mid-stage luteal cells produced more P4 than late-stage cells; regardless of stage, P4 decreased with time. The time x LH interaction (P < .001) revealed that at 6 and 12 h the 50- and 100-ng doses of LH increased P4 to greater than the 0-ng dose. Production of PGF2 alpha by mid-stage cells was similar among the three PSPB treatments; however, PGF2 alpha production by late-stage cells increased (P < .01) in response to the 5.0-micrograms dose of PSPB. The LH did not affect PGF2 alpha production. Late-stage luteal cells produced more (P < .001) PGF2 alpha than mid-stage cells during the 18-h pretreatment period and at 6, but not 12, h.(ABSTRACT TRUNCATED AT 250 WORDS)
R P Del Vecchio; W D Sutherland; R G Sasser
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of animal science     Volume:  73     ISSN:  0021-8812     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  1995 Sep 
Date Detail:
Created Date:  1996-03-21     Completed Date:  1996-03-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2662-8     Citation Subset:  IM    
Agriculture & Agri-Food Canada Research Centre, Brandon, Manitoba.
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MeSH Terms
Aspartic Acid Endopeptidases / pharmacology*
Cattle / metabolism*,  physiology
Cells, Cultured
Dinoprost / metabolism
Dinoprostone / metabolism
Estrus / metabolism*,  physiology
Luteal Cells / cytology,  drug effects,  metabolism*
Luteinizing Hormone / pharmacology
Oxytocin / metabolism*
Pregnancy Proteins / pharmacology*
Progesterone / metabolism*
Prostaglandins / metabolism*
Reg. No./Substance:
0/Pregnancy Proteins; 0/Prostaglandins; 363-24-6/Dinoprostone; 50-56-6/Oxytocin; 551-11-1/Dinoprost; 57-83-0/Progesterone; 9002-67-9/Luteinizing Hormone; EC 3.4.23.-/Aspartic Acid Endopeptidases; EC 3.4.23.-/pregnancy-associated glycoprotein 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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