Document Detail


Effect of pregnancy on the roles of nitric oxide and prostaglandins in 5-hydroxytryptamine-induced contractions in rat isolated thoracic and abdominal aorta.
MedLine Citation:
PMID:  15743404     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. Vascular resistance and sensitivity to circulating pressor and vasoconstrictor substances are blunted during pregnancy. This has been attributed mainly to an increased production of endothelium-derived mediators. The aim of the present study was to determine whether pregnancy changes the relative participation of nitric oxide (NO) and prostaglandins (PG) in the modulation of the contractile response to 5-hydroxytryptamine (5-HT) in two anatomically distint segments of the rat aorta. 2. Full concentration-response curves to 5-HT were obtained in isolated rings from the thoracic and abdominal portion of the aorta from pregnant and non-pregnant rats in the presence and absence of the NO synthase (NOS) inhibitor N(G)-nitro-l-arginine methyl ester (L-NAME; 10 micromol/L) or the PG synthesis inhibitor indomethacin (10 micromol/L). Cyclo-oxygenase (COX)-1, COX-2 and endothelial (e) NOS protein expression were determined in the same tissues by immunoblot. 3. The effects of pregnancy were accentuated in the abdominal compared with the thoracic aorta. In addition, the relative participation of the NO and PG pathways seems to be changed during pregnancy. Although NO seems to be the mediator mainly responsible for the effect of pregnancy in the thoracic aorta, our results suggest a complex interaction between NO and PG in the abdominal aorta. Indomethacin significantly reduced the contractile response of both segments of the aorta, whereas expression of COX-1, COX-2 and eNOS were increased only in the abdominal segment of pregnant animals. 4. These results show that the effect of pregnancy is not homogeneous along the aorta. There seems to be a mutual interaction between PG and NO in the abdominal, but not in the thoracic, aorta from pregnant rats: the role of NO becomes evident in the absence of vasodilatory PG, whereas the participation of the latter increases in the absence of NO working as a compensatory mechanism.
Authors:
Rosa A Bobadilla L; Víctor Pérez-Alvarez; Ismael Bracho Valdés; Pedro López-Sanchez
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental pharmacology & physiology     Volume:  32     ISSN:  0305-1870     ISO Abbreviation:  Clin. Exp. Pharmacol. Physiol.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-03     Completed Date:  2005-06-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0425076     Medline TA:  Clin Exp Pharmacol Physiol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  202-9     Citation Subset:  IM    
Affiliation:
Departamento de Fisiología y Farmacología, Escuela Superior de Medicina del IPN, Plan de San Luis y Diaz Mirón, Casco de Santo Tomás, México. rabobadilla@terra.com.mx
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta, Abdominal / physiology*
Aorta, Thoracic / physiology*
Cyclooxygenase 1
Cyclooxygenase 2
Female
Membrane Proteins
Muscle Contraction
Muscle, Smooth, Vascular / physiology*
Nitric Oxide / physiology*
Nitric Oxide Synthase / antagonists & inhibitors,  biosynthesis
Nitric Oxide Synthase Type III
Pregnancy
Pregnancy, Animal / metabolism,  physiology*
Prostaglandin Antagonists / pharmacology
Prostaglandin-Endoperoxide Synthases / biosynthesis
Prostaglandins / biosynthesis,  physiology*
Rats
Rats, Wistar
Serotonin / pharmacology
Vasoconstrictor Agents / pharmacology
Chemical
Reg. No./Substance:
0/Membrane Proteins; 0/Prostaglandin Antagonists; 0/Prostaglandins; 0/Vasoconstrictor Agents; 10102-43-9/Nitric Oxide; 50-67-9/Serotonin; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.14.13.39/Nos3 protein, rat; EC 1.14.99.1/Cyclooxygenase 1; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases; EC 1.14.99.1/Ptgs1 protein, rat

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