Document Detail

Effect of pregnancy and feeding level on cell turnover and expression of related genes in the mammary tissue of lactating dairy cows.
MedLine Citation:
PMID:  22443574     Owner:  NLM     Status:  In-Data-Review    
Milk yield is reduced by pregnancy, and the present experiment was conducted to study the biological basis for the negative effect of pregnancy on milk yield. A total of 16 dairy cows were fed at either a normal or a low feeding level (eight cows per treatment), and half of them were inseminated after approximately 3 months of lactation and the other half were not inseminated. Mammary biopsies were taken at approximately 9 months of lactation. The milk yield of pregnant cows was reduced by 2.6 kg/day, and lactation persistency was reduced already from the time of insemination. Low feeding level reduced the milk yield by 9.8 kg/day from week 8 to week 39 of lactation, whereas no interaction between pregnancy and feeding level was found. Cell proliferation (Ki-67) and apoptosis (terminal deoxynucleotidyl transferase dUTP nick end labeling, TUNEL) were unaffected by feeding level, and pregnancy tended to reduce cell proliferation but had no effect on apoptosis. Reduced cell proliferation may explain the reduced lactation persistency in pregnant cows. Transcription of oestrogen receptor α, progesterone receptor A and B, and long and short isoforms of the prolactin receptor were higher in pregnant cows compared with non-pregnant cows. Feeding level did not mediate changes in transcription of genes. Transcription of other cell-turnover-related genes (IGF-I, IGF binding protein-5, caspase-3) as well as genes related to the secretory activity of the cells (α-lactalbumin and acetyl CoA carboxylase α) was not affected by pregnancy or by feeding level.
J V Nørgaard; M T Sørensen; P K Theil; J Sehested; K Sejrsen
Related Documents :
20572114 - The making of fetal surgery.
24811064 - Usefulness of circulating micrornas for the prediction of early preeclampsia at first-t...
22443574 - Effect of pregnancy and feeding level on cell turnover and expression of related genes ...
6740334 - Fetal compensatory responses to reduced oxygen delivery.
19622204 - The safety of anti-epileptic drug regimens: a qualitative study of factors determining ...
17762534 - Coagulation factor deficiency as a cause of recurrent fetal loss: a red herring!
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Animal : an international journal of animal bioscience     Volume:  2     ISSN:  1751-7311     ISO Abbreviation:  Animal     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2012-03-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101303270     Medline TA:  Animal     Country:  England    
Other Details:
Languages:  eng     Pagination:  588-94     Citation Subset:  -    
Department of Animal Health, Welfare and Nutrition, University of Aarhus, P.O. Box 50, DK-8830 Tjele, Denmark.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Plasma concentration of leptin and ghrelin in Standardbred foals as related to the age, sex, exercis...
Next Document:  Osmolarity and composition of cell culture media affect further development and survival in zebrafis...