Document Detail


Effect of pregnancy and collection technique on prostaglandin F in the uterine lumen of Pony mares.
MedLine Citation:
PMID:  6962869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Uterine flushings were obtained through the cervix (Method A) and through the wall of the uterus after hysterectomy (Method B) of ovariectomized Pony mares after s.c. injection of oestrogen for 1 week and progesterone for 2 weeks (Exp. 1). Non-pregnant and pregnant mares were flushed by Method A on Day 14 after ovulation and the flushings compared with those of non-pregnant mares injected i.v. with flunixen meglumine, a prostaglandin synthetase inhibitor, shortly before flushing (Exp. 3). Uterine flushings were also collected by Methods A and B from non-pregnant and pregnant Pony mares on Day 14. Endometrial and embryonic tissues from these mares were incubated with and without flunixen meglumine (Exp. 3). In all experiments, pregnancy had a significant effect on PGF content of uterine flushings or incubation media. Flushings from pregnant mares had reduced levels of PGF and were not influenced by collection technique (Exps 1 & 3). Non-pregnant Pony mares treated with progesterone responded to cervical stimulation (Method A) with an increase in intrauterine PGF over levels measured after hysterectomy (Method B) (Exps 1, 2 & 3). There was no effect on endometrial production of PGF in vitro by any tissue combination in a 2 h incubation in Krebs-Ringer-bicarbonate buffer but after 12 h incubation in Minimum Essential Medium endometrial PGF production was significantly higher when the endometria were from pregnant mares than from non-pregnant mares. PGF production in vitro was significantly suppressed by flunixen meglumine, by yolk sac membranes, and yolk sac and trophoblast, but not by trophoblast alone. The low intrauterine PGF levels in pregnant mares and the low in-vitro PGF production in the presence of the conceptus membranes may reflect inhibition of PGF synthesis and/or release by the embryo.
Authors:
L A Berglund; D C Sharp; M W Vernon; W W Thatcher
Related Documents :
16112529 - French field results (1985-2005) on factors affecting fertility of frozen stallion semen.
23772229 - Indigenous women of latin america: unintended pregnancy, unsafe abortion, and reproduct...
10596929 - Transabdominal ultrasonographic determination of fetal gender in the horse during mid-g...
24006559 - Unintended pregnancy and unsafe abortion in the philippines: context and consequences.
3199369 - Reproduction in female hartmann's zebra, equus zebra hartmannae.
23524529 - Preservation of sperm of cancer patients: extent of use and pregnancy outcome in a tert...
21820259 - Cervical ectopic pregnancy diagnosed by point-of-care emergency department ultrasound.
17010559 - Production of inhibin a and inhibin b in boars: changes in testicular and circulating l...
1948589 - The role of cervical cerclage in the management of uterine anomalies.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of reproduction and fertility. Supplement     Volume:  32     ISSN:  0449-3087     ISO Abbreviation:  J. Reprod. Fertil. Suppl.     Publication Date:  1982  
Date Detail:
Created Date:  1983-05-27     Completed Date:  1983-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0225652     Medline TA:  J Reprod Fertil Suppl     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  335-41     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Castration
Clonixin / analogs & derivatives,  pharmacology
Estrogens / pharmacology
Female
Horses / physiology*
Hysterectomy
Pregnancy
Pregnancy, Animal*
Progesterone / pharmacology
Prostaglandins F / isolation & purification*
Uterus / drug effects,  physiology*
Chemical
Reg. No./Substance:
0/Estrogens; 0/Prostaglandins F; 17737-65-4/Clonixin; 42461-84-7/flunixin meglumine; 57-83-0/Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Transmission electron microscopy of horse embryos 3-16 days after ovulation.
Next Document:  Studies on oestrogen synthesis by the preimplantation equine conceptus.