| Effect of prebiotic fibre supplementation on hepatic gene expression and serum lipids: a dose-response study in JCR:LA-cp rats. | |
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MedLine Citation:
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PMID: 20021705 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Prebiotic fibres have been proposed to promote weight loss and lower serum cholesterol; however, the mechanisms are not fully understood. The aim of the present research was to identify possible mechanisms through which prebiotic fibres improve serum lipids. Lean and obese JCR:La-cp rats aged 8 weeks consumed one of three diets supplemented with 0, 10 or 20 % prebiotic fibre for 10 weeks. Rats were anaesthetised and a fasting blood sample was taken for lipid analysis. Real-time PCR was used to determine gene expression for cholesterol and fatty acid regulatory genes in liver tissue. Liver and caecal digesta cholesterol and TAG content were quantified. Both doses of prebiotic fibre lowered serum cholesterol levels by 24 % in the obese hyperlipidaemic rats (P < 0.05). This change was associated with an increase in caecal digesta as well as an up-regulation of genes involved in cholesterol synthesis and bile production. Additionally, there was a 42 % reduction in TAG accumulation in the liver of the obese rats with 10 % prebiotic diet (P < 0.05); however, no change in liver fatty acid synthase (FAS). Prebiotic fibres appear to lower cholesterol levels through increased cholesterol excretion in the form of bile and inhibit the accumulation of TAG in the liver through a mechanism unrelated to FAS. These effects appear to be limited to the obese model and particularly the 10 % dose. The present work is significant as it provides insight into the mechanisms of action for prebiotic fibres on lipid metabolism and furthers the development of dietary treatments for hypercholesterolaemia. |
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Authors:
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Jill A Parnell; Raylene A Reimer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-21 |
Journal Detail:
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Title: The British journal of nutrition Volume: 103 ISSN: 1475-2662 ISO Abbreviation: Br. J. Nutr. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-03 Completed Date: 2010-06-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372547 Medline TA: Br J Nutr Country: England |
Other Details:
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Languages: eng Pagination: 1577-84 Citation Subset: IM |
Affiliation:
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Department of Medical Sciences, Faculty of Medicine, University of Calgary, 2500 University Drive NW, Calgary, AB, Canada T2N 1N4. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetyl-CoA Carboxylase
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genetics Animals Cecum / chemistry Cholesterol / analysis, genetics Cholesterol 7-alpha-Hydroxylase / genetics Dietary Fiber / administration & dosage* Dose-Response Relationship, Drug Fatty Acid Synthetase Complex / genetics Fatty Acids / genetics Gene Expression* Hydroxymethylglutaryl CoA Reductases / genetics Inulin / administration & dosage Lipids / blood* Liver / chemistry, enzymology, metabolism* Male Obesity / metabolism* Oligosaccharides / administration & dosage Polymerase Chain Reaction Prebiotics* RNA, Messenger / analysis Rats Sterol Regulatory Element Binding Protein 1 / genetics Triglycerides / analysis |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Lipids; 0/Oligosaccharides; 0/Prebiotics; 0/RNA, Messenger; 0/Sterol Regulatory Element Binding Protein 1; 0/Triglycerides; 0/oligofructose; 57-88-5/Cholesterol; 9005-80-5/Inulin; EC 1.1.1.-/Hydroxymethylglutaryl CoA Reductases; EC 1.14.13.17/Cholesterol 7-alpha-Hydroxylase; EC 6.-/Fatty Acid Synthetase Complex; EC 6.4.1.2/Acetyl-CoA Carboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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