Document Detail

Effect of potassium sorbate on cellular GSH level and lipid peroxidation in cultured rat hepatocytes.
MedLine Citation:
PMID:  9635512     Owner:  NLM     Status:  MEDLINE    
Change in cellular reduced glutathione (GSH) level was examined after the addition of 1-10 mM potassium sorbate (SA-K) to cultured rat hepatocytes. The cellular GSH content was decreased to the lowest level at 6 h after the addition of SA-K, and then gradually returned to the normal level except for hepatocytes exposed to 10 mM SA-K. Although the decrease in GSH level was not associated with lactate dehydrogenase (LDH) leakage in hepatocytes exposed to SA-K up to the concentration of 5 mM, cell injury was caused in cells exposed to 10 mM SA-K. When eicosapentaenoic acid was added in conjunction with various concentrations of SA-K to hepatocytes, peroxidation of the fatty acid was accelerated in parallel with the decrease in cellular GSH level. The enhanced lipid peroxidation in the hepatocytes co-exposed to SA-K and eicosapentaenoic acid (EPA) induced the development of cell injury. These results suggest that hepatocytes exposed to SA-K become susceptible to oxidative stress such as lipid peroxidation.
N Sugihara; Y Tsuruta; K Furuno
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  21     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-08-24     Completed Date:  1998-08-24     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  524-6     Citation Subset:  IM    
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan.
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MeSH Terms
Antioxidants / pharmacology
Cells, Cultured
Eicosapentaenoic Acid / metabolism
Glutathione / metabolism*
L-Lactate Dehydrogenase / metabolism
Lipid Peroxidation / drug effects*
Liver / cytology,  drug effects,  metabolism*
Malondialdehyde / metabolism
Rats, Wistar
Sorbic Acid / pharmacology*
Reg. No./Substance:
0/Antioxidants; 110-44-1/Sorbic Acid; 1553-41-9/Eicosapentaenoic Acid; 542-78-9/Malondialdehyde; 70-18-8/Glutathione; EC Dehydrogenase

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