| Effect of postmortem storage on the Z-line region of titin in bovine muscle. | |
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MedLine Citation:
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PMID: 9581927 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Myofibrils were prepared from bovine muscles (cutaneous trunci, rectus abdominis, psoas major, and masseter) and compared between different aging periods at 4 degrees C (0, 1, 2, 4, 8, and 16 d). Myofibrils were stained with an antibody directed against a 56-kDa fragment (FE-RE) of titin located in the Z-line region. Unaged myofibrils from all four muscles showed a single stained band at the Z-line with similar intensities. Postmortem time did not significantly affect the total amount of fluorescence in the sarcomere, suggesting the titin FE-RE epitope was not degraded nor were titin fragments containing this epitope released during storage. However, the fluorescence patterns were altered. The relative fluorescence intensity at the Z-line decreased but that in the I-band increased gradually, showing the translocation of some titin FE-RE epitopes during the aging period. This suggested that a cleavage occurred in a region of titin very close to the Z-line during postmortem storage. Usually the position of maximum fluorescence remained at the Z-line, although about 1/3 of the myofibrils from rectus abdominis showed a two-band pattern around the Z-line after 16 d of aging. The titin changes observed may be related to the increased fragility of the myofibril and the improvement of meat tenderness during postmortem storage. |
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Authors:
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C Boyer-Berri; M L Greaser |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of animal science Volume: 76 ISSN: 0021-8812 ISO Abbreviation: J. Anim. Sci. Publication Date: 1998 Apr |
Date Detail:
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Created Date: 1998-06-29 Completed Date: 1998-06-29 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8003002 Medline TA: J Anim Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1034-44 Citation Subset: IM |
Affiliation:
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Muscle Biology Laboratory, University of Wisconsin-Madison, 53706, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Chickens Epitopes / immunology Immune Sera / immunology Male Masseter Muscle / immunology, pathology Mice Muscle Proteins / analysis* Muscle, Skeletal / immunology, pathology* Myofibrils / chemistry*, immunology Postmortem Changes* Protein Kinases / analysis* Psoas Muscles / immunology, pathology Rabbits Rectus Abdominis / immunology, pathology Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Epitopes; 0/Immune Sera; 0/Muscle Proteins; 0/connectin; EC 2.7.-/Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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