Document Detail


Effect of postmortem storage on the Z-line region of titin in bovine muscle.
MedLine Citation:
PMID:  9581927     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Myofibrils were prepared from bovine muscles (cutaneous trunci, rectus abdominis, psoas major, and masseter) and compared between different aging periods at 4 degrees C (0, 1, 2, 4, 8, and 16 d). Myofibrils were stained with an antibody directed against a 56-kDa fragment (FE-RE) of titin located in the Z-line region. Unaged myofibrils from all four muscles showed a single stained band at the Z-line with similar intensities. Postmortem time did not significantly affect the total amount of fluorescence in the sarcomere, suggesting the titin FE-RE epitope was not degraded nor were titin fragments containing this epitope released during storage. However, the fluorescence patterns were altered. The relative fluorescence intensity at the Z-line decreased but that in the I-band increased gradually, showing the translocation of some titin FE-RE epitopes during the aging period. This suggested that a cleavage occurred in a region of titin very close to the Z-line during postmortem storage. Usually the position of maximum fluorescence remained at the Z-line, although about 1/3 of the myofibrils from rectus abdominis showed a two-band pattern around the Z-line after 16 d of aging. The titin changes observed may be related to the increased fragility of the myofibril and the improvement of meat tenderness during postmortem storage.
Authors:
C Boyer-Berri; M L Greaser
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of animal science     Volume:  76     ISSN:  0021-8812     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-06-29     Completed Date:  1998-06-29     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1034-44     Citation Subset:  IM    
Affiliation:
Muscle Biology Laboratory, University of Wisconsin-Madison, 53706, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Chickens
Epitopes / immunology
Immune Sera / immunology
Male
Masseter Muscle / immunology,  pathology
Mice
Muscle Proteins / analysis*
Muscle, Skeletal / immunology,  pathology*
Myofibrils / chemistry*,  immunology
Postmortem Changes*
Protein Kinases / analysis*
Psoas Muscles / immunology,  pathology
Rabbits
Rectus Abdominis / immunology,  pathology
Time Factors
Chemical
Reg. No./Substance:
0/Epitopes; 0/Immune Sera; 0/Muscle Proteins; 0/connectin; EC 2.7.-/Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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