Document Detail

Effect of pore size and interpore distance on endothelial cell growth on polymers.
MedLine Citation:
PMID:  18200559     Owner:  NLM     Status:  MEDLINE    
The endothelization of polymers using surface modification has received great attention. In particular, creation of physical surface features such as craters or pores has been an active area of research. However, there have been no reported studies of the effects of pore sizes (wide range) and interpore distance on endothelial cell growth. This report details the study done on endothelial cell attachment on the surfaces of polymers modified by porogen leaching. The polymeric system studied includes PLLA and PLGA (80/20). Factors such as porogen type, pore size, and interpore distance were varied, and the surface was evaluated for its influence on endothelial cell growth. Three groups of pore sizes were evaluated: small (5-20 mum), medium (20-45 mum), and large pores (45-90 mum). Two porogens were evaluated: sugar and gelatin. In addition to counting the attached endothelial cells, their proliferation was also quantified. Pore size and interpore distances were evaluated using scanning electron microscopy (SEM), and cell morphology was studied by staining with crystal violet. Analysis of variance demonstrated that the main parameters, pore size and interpore distance were significant in endothelial cell growth. In PLGA (80/20), it was found that endothelial cell growth was enhanced by smaller pore size and lower interpore distance, whereas the growth was poor on PLLA regardless of pore features.
D Narayan; S S Venkatraman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  87     ISSN:  1552-4965     ISO Abbreviation:  -     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-03     Completed Date:  2009-01-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  710-8     Citation Subset:  IM    
Copyright Information:
(c) 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2008.
School of Materials Science and Engineering, Nanyang Technological University, Blk N4.1-02-06, 50, Nanyang Avenue, Singapore 637819.
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MeSH Terms
Analysis of Variance
Biocompatible Materials / chemistry*
Cell Proliferation
Cells, Cultured
Endothelial Cells / physiology*
Gelatin / metabolism
Lactic Acid / chemistry*
Microscopy, Electron, Scanning
Polyglycolic Acid / chemistry*
Polymers / chemistry*
Sucrose / metabolism
Surface Properties
Reg. No./Substance:
0/Biocompatible Materials; 0/Polymers; 0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 26100-51-6/poly(lactic acid); 50-21-5/Lactic Acid; 57-50-1/Sucrose; 9000-70-8/Gelatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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