| Effect of polyunsaturated fatty acids on endocannabinoid and N-acyl-ethanolamine levels in mouse adipocytes. | |
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MedLine Citation:
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PMID: 18054340 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The tissue concentrations of the endocannabinoids, 2-arachidonoylglycerol (2-AG) and N-arachidonoyl-ethanolamine (anandamide), are altered in the adipose tissue of mice fed a high fat diet. We have investigated here the effect on endocannabinoid levels of incubation of mouse 3T3-F442A adipocytes with several free polyunstaurated fatty acids (PUFAs), including linolenic acid (LA), alpha-linolenic acid (ALA), arachidonic acid (AA) and docosahexaenoic acid (DHA), as well as oleic acid (OA) and palmitic acid (PA). By using mass spectrometric methods, we quantified the levels of endocannabinoids, of two anandamide congeners, N-palmitoyl-ethanolamine (PEA) and N-oleoyl-ethanolamine (OEA), and of fatty acids esterified in triacylglycerols or phospholipids, which act as 2-AG and/or N-acyl-ethanolamine precursors. Incubation with AA strongly elevated 2-AG levels and the amounts of AA esterified in triacylglycerols and on glycerol carbon atom 2 (sn-2), but not 1 (sn-1), in phospholipids. Incubation with DHA decreased 2-AG and anandamide levels and the amounts of AA esterified on both the sn-2 and sn-1 position of phospholipids, but not on triacylglycerols. PEA levels augmented following incubation of adipocytes with OA and PA, with no corresponding changes in phospholipids and triacylglycerols. We suggest that dietary PUFAs might modulate the levels of adipocyte phospholipids that act as endocannabinoid precursors. |
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Authors:
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Isabel Matias; Gianfranca Carta; Elisabetta Murru; Stefania Petrosino; Sebastiano Banni; Vincenzo Di Marzo |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-11-17 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1781 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2008 Jan-Feb |
Date Detail:
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Created Date: 2008-02-11 Completed Date: 2008-04-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 52-60 Citation Subset: IM |
Affiliation:
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Endocannabinoid Research Group, Institute of Biomolecular Chemistry, Consiglio Nazionale delle Ricerche, via dei Campi Flegrei 34, 80078 Pozzuoli (Napoli), Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes
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drug effects*,
metabolism* Animals Cell Line Endocannabinoids / metabolism* Ethanolamines / metabolism* Fatty Acids, Unsaturated / pharmacology* Mice Triglycerides / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Endocannabinoids; 0/Ethanolamines; 0/Fatty Acids, Unsaturated; 0/N-acylethanolamines; 0/Triglycerides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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