Document Detail


Effect of polyphenols on enniatins-induced cytotoxic effects in mammalian cells.
MedLine Citation:
PMID:  22888764     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Enniatins (ENs) are fungal secondary metabolites produced by genus Fusarium. The ENs exert antimicrobial and insecticidal effect, and has also been demonstrated cytotoxic effects on several mammalian cell lines. On the other hands, it has been proved that natural polyphenols have antioxidant effect. In this study, cell effects at low levels of exposure of four ENs (A, A(1), B and B(1)) and five polyphenols (quercetin, quercetin-3-β-D-glucoside, rutin, myricetin and t-pterostilbene) present in wine; and the cytoprotective effect of these polyphenols exposed simultaneously with ENs in Chinese Hamster Ovary (CHO-K1) cells, were studied. Cell effects were determined by the MTT test after 24 h of exposure. All ENs showed cytotoxic effect. The IC(50) obtained ranged from 4.5 ± 1.2 to 11.0 ± 2.7 M. The concentration of polyphenols tested ranged from 5 to 50 µM. Polyphenols did not show cytotoxicity and the cytoprotective effect of polyphenols varies depending on the EN tested. The cytoprotective effect of polyphenols in CHO-K1 cells exposed to ENs was as follow: quercetin, from 24 to 84%; quercetin-3-D-glucoside, from 12 to 76%; rutin, from 17 to 83%; myricetin, from 16 to 92% and pterostilbene from 25 to 100%. All polyphenols protected CHO-K1 cells against EN A(1) exposure.
Authors:
G Lombardi; A Prosperini; G Font; M J Ruiz
Related Documents :
22833314 - Glycosphingolipid composition of epithelial cells isolated along the villus axis of sma...
21275264 - Tamoxifen enhances the anti-proliferative effect of roscovitine, a selective cyclin-dep...
21029414 - Cell-free mirnas may indicate diagnosis and docetaxel sensitivity of tumor cells in mal...
21296134 - Aflatoxin b1 - a potential endocrine disruptor - up-regulates cyp19a1 in jeg-3 cells.
21372494 - Effect of histone deacetylase inhibitor in combination with 5-fluorouracil on pancreas ...
25395694 - Synthesis and cell phototoxicity of a triply bridged fused diporphyrin appended with si...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-14
Journal Detail:
Title:  Toxicology mechanisms and methods     Volume:  -     ISSN:  1537-6524     ISO Abbreviation:  Toxicol. Mech. Methods     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134521     Medline TA:  Toxicol Mech Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Dipartmento di Scienze Economico-Estimative e degli Alimenti, Sezione di Chimica Bromatologica, Biochimica, Fisiologia e Nutrizione, Facoltà di Farmacia. Università degli Studi di Perugia, via San Costanzo (interno orto botanico) 06126, Perugia, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Review: a meta-analysis of GWAS and age-associated diseases.
Next Document:  Enhancing the power of liquid chromatography-mass spectrometry- based urine metabolomics in negative...