Document Detail

Effect of the polyene antibiotic filipin on the permeability of the inward- and the outward-facing membranes of the isolated from skin (Rana temporaria).
MedLine Citation:
PMID:  300979     Owner:  NLM     Status:  MEDLINE    
The effect of the polyene antibiotic filipin on the electrical properties and the passive permeability of the cell membranes was investigated. The addition of filipin to the outside bathing solution has the following effects: 1. it results in a drastic reduction in the transepithelial resistance and potential, 2. it causes a 10-20 times increase in the passive transepithelial chloride, sodium and sucrose flux, 3. it results in the formation of an amiloride insensitive sodium pathway in the outward facing membrane, 4. it results in an active outward transport of potassium, 5. it results in a highly significant swelling of all the cells in the epithelium. The addition of filipin to the inside bathing solution has the following effects 1. it results in an activation of the active sodium transport, 2. it causes a slight increase in the passive transepithelial chloride and sodium permeabilities but has no effect on the sucrose permeability, 3. it has no effect on the amiloride inhibition of the short-circuit current, 4. it has no effect on the volume of the cells in the epithelium. It is suggested that the addition of filipin to the outside bathing solution increases the direct sodium flow from cell to cell in neighbour layers. Furthermore these experiments indicate that the outward facing membrane of the isolated frog skin has a high cholesterol content as compared with the cholesterol content of the inward facing membrane.
R Nielsen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta physiologica Scandinavica     Volume:  99     ISSN:  0001-6772     ISO Abbreviation:  Acta Physiol. Scand.     Publication Date:  1977 Apr 
Date Detail:
Created Date:  1977-06-22     Completed Date:  1977-06-22     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370362     Medline TA:  Acta Physiol Scand     Country:  SWEDEN    
Other Details:
Languages:  eng     Pagination:  399-411     Citation Subset:  IM    
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MeSH Terms
Amiloride / pharmacology
Amphotericin B / pharmacology
Cell Membrane Permeability / drug effects*
Chlorides / metabolism
Drug Combinations
Filipin / pharmacology*
Polyenes / pharmacology*
Rana temporaria
Skin / cytology,  drug effects*
Sodium / metabolism
Sucrose / metabolism
Time Factors
Reg. No./Substance:
0/Chlorides; 0/Drug Combinations; 0/Polyenes; 1397-89-3/Amphotericin B; 2609-46-3/Amiloride; 480-49-9/Filipin; 57-50-1/Sucrose; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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