| Effect of polyamines on isoleucyl-tRNA formation by rat-liver isoleucyl-tRNA synthetase. | |
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MedLine Citation:
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PMID: 244419 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of polyamines on rat-liver isoelucyl-tRNA formation was studied using isoleucyl-tRNA synthetase purified by column chromatography successively on Sephadex G-200, DEAE-Sephadex A-25, and tRNA-Sepharose 4B. In the presence of 50 mMK+, isoleucyl-tRNA formation was inhibited markedly by 1.5 mM or higher concentrations of Mg2+. However, the addition of spermine to the reaction mixture prevented the inhibitory effect of Mg2+. In the presence of 200 mMK+, the addition of spermine to the reaction mixture stimulated isoleucyl-tRNA formation in the presence of Mg2+ concentrations from 0 to 5 mM. Although the effective concentration was different, spermidine exhibited a similar stimulative effect. The effective concentration of spermine required for stimulation was higher when larger amounts of tRNA were used. The stimulatory effect of isoleucyl-tRNA formation by polyamines was shown to reflect on polypeptide synthesis. When formaldehyde-treated poly(A,U) was used as messenger RNA, polypeptide synthesis from amino acids was stimulated by polyamines, but that from aminoacyl-tRNAs was not stimulated by polyamines. |
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Authors:
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K Igarashi; K Eguchi; M Tanaka; S Hirose |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 82 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1978 Jan |
Date Detail:
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Created Date: 1978-03-29 Completed Date: 1978-03-29 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 301-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acyl-tRNA Synthetases
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metabolism* Animals Isoleucine-tRNA Ligase / isolation & purification, metabolism* Kinetics Liver / enzymology* Magnesium / pharmacology Putrescine / pharmacology* RNA, Transfer, Amino Acyl / biosynthesis* Rats Ribosomes / metabolism Spermidine / pharmacology* Spermine / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Transfer, Amino Acyl; 110-60-1/Putrescine; 124-20-9/Spermidine; 71-44-3/Spermine; 7439-95-4/Magnesium; EC 6.1.1.-/Amino Acyl-tRNA Synthetases; EC 6.1.1.5/Isoleucine-tRNA Ligase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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